Chromatin Immunoprecipitation Sequencing Technology Reveals Global Regulatory Roles of Low-Cell-Density Quorum-Sensing Regulator AphA in the Pathogen Vibrio Alginolyticus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2016 Aug 24

PMID 27551022

Citations 13

Authors

Dan Gu

Huan Liu

Zhen Yang

Yuanxing Zhang

Qiyao Wang

Affiliations

Soon will be listed here.

Abstract

Importance: In this work, we determined that AphA, the master regulator of QS at low cell density, plays essential roles in expression of genes associated with physiology and virulence in V. alginolyticus, a Gram-negative pathogen for humans and marine animals. We further uncovered that 49 genes could be directly regulated by AphA and a 19-bp consensus binding sequence was identified. Among the 49 genes, the QS and other non-QS-associated genes were identified to be regulated by AphA. Besides, the small RNA CsrB was negatively regulated by AphA, and AphA regulate motility abilities through both CsrB and LuxR. Taken together, the findings of this study improve our understanding of the complex regulation network of AphA and QS.

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