SGO1 is Involved in the DNA Damage Response in MYCN-amplified Neuroblastoma Cells
Authors
Affiliations
Shugoshin 1 (SGO1) is required for accurate chromosome segregation during mitosis and meiosis; however, its other functions, especially at interphase, are not clearly understood. Here, we found that downregulation of SGO1 caused a synergistic phenotype in cells overexpressing MYCN. Downregulation of SGO1 impaired proliferation and induced DNA damage followed by a senescence-like phenotype only in MYCN-overexpressing neuroblastoma cells. In these cells, SGO1 knockdown induced DNA damage, even during interphase, and this effect was independent of cohesin. Furthermore, MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. Therefore, we propose that SGO1 represents a potential molecular target for treatment of MYCN-amplified neuroblastoma.
Shugoshin 1 expression in various cancers: a potential target for therapy.
Ankathatti Narayanaswamy I, Kattepur A, Raju K, Perumal V, Ramalingam R, Raavi V Clin Transl Oncol. 2024; .
PMID: 39476301 DOI: 10.1007/s12094-024-03749-1.
Mariani J, Mansky B, Madsen P, Salinas D, Kesmen D, Huynh N Nat Commun. 2024; 15(1):3873.
PMID: 38719882 PMC: 11079006. DOI: 10.1038/s41467-024-48118-2.
Yang Z, Jiang Y, Wang L, Yu B, Cai H, Fan J BMC Med Genomics. 2024; 17(1):60.
PMID: 38383432 PMC: 10882763. DOI: 10.1186/s12920-024-01825-7.
Shift of N-MYC Oncogene Expression in AML Patients Carrying the FLT3-ITD Mutation.
Bogdanov K, Kudryavtseva E, Fomicheva Y, Churkina I, Lomaia E, Girshova L Pathophysiology. 2023; 30(3):296-313.
PMID: 37606386 PMC: 10443239. DOI: 10.3390/pathophysiology30030024.
A Review of the Regulatory Mechanisms of N-Myc on Cell Cycle.
Li H, Dong L, Jin M, Li Q, Wang X, Jia M Molecules. 2023; 28(3).
PMID: 36770809 PMC: 9920120. DOI: 10.3390/molecules28031141.