Blood Stage Plasmodium Falciparum Exhibits Biological Responses to Direct Current Electric Fields

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Aug 19

PMID 27537497

Citations 6

Authors

Lorena M Coronado

Stephania Montealegre

Zumara Chaverra

Luis Mojica

Carlos Espinosa

Alejandro Almanza

Ricardo Correa

Jose A Stoute

Rolando A Gittens

Carmenza Spadafora

Affiliations

Soon will be listed here.

Abstract

The development of resistance to insecticides by the vector of malaria and the increasingly faster appearance of resistance to antimalarial drugs by the parasite can dangerously hamper efforts to control and eradicate the disease. Alternative ways to treat this disease are urgently needed. Here we evaluate the in vitro effect of direct current (DC) capacitive coupling electrical stimulation on the biology and viability of Plasmodium falciparum. We designed a system that exposes infected erythrocytes to different capacitively coupled electric fields in order to evaluate their effect on P. falciparum. The effect on growth of the parasite, replication of DNA, mitochondrial membrane potential and level of reactive oxygen species after exposure to electric fields demonstrate that the parasite is biologically able to respond to stimuli from DC electric fields involving calcium signaling pathways.

Citing Articles

Electric stimulation: a versatile manipulation technique mediated microbial applications.

Adhikari M, Wang L, Adhikari D, Khadka S, Ullah M, Mbituyimana B Bioprocess Biosyst Eng. 2024; 48(2):171-192.

PMID: 39611964 DOI: 10.1007/s00449-024-03107-z.

Biogenically synthesized green silver nanoparticles exhibit antimalarial activity.

Tiwari S, Kumar R, Devi S, Sharma P, Chaudhary N, Negi S Discov Nano. 2024; 19(1):136.

PMID: 39217276 PMC: 11365884. DOI: 10.1186/s11671-024-04098-2.

A low-cost, portable device for the study of the malaria parasite's growth inhibition via microwave exposure.

Rua E, Coronado L, Donado Morcillo C, Correa R, Solis L, Spadafora C HardwareX. 2024; 19:e00540.

PMID: 38988372 PMC: 11234006. DOI: 10.1016/j.ohx.2024.e00540.

Beyond schistosomiasis: unraveling co-infections and altered immunity.

Perera D, Koger-Pease C, Paulini K, Daoudi M, Ndao M Clin Microbiol Rev. 2024; 37(1):e0009823.

PMID: 38319102 PMC: 10938899. DOI: 10.1128/cmr.00098-23.

Microwaves can kill malaria parasites non-thermally.

Coronado L, Stoute J, Nadovich C, Cheng J, Correa R, Chaw K Front Cell Infect Microbiol. 2023; 13:955134.

PMID: 36816585 PMC: 9932958. DOI: 10.3389/fcimb.2023.955134.

References

Brighton C, Wang W, Seldes R, Zhang G, Pollack S . Signal transduction in electrically stimulated bone cells. J Bone Joint Surg Am. 2001; 83(10):1514-23. DOI: 10.2106/00004623-200110000-00009. View

Corbett Y, Herrera L, Gonzalez J, Cubilla L, Capson T, Coley P . A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity. Am J Trop Med Hyg. 2004; 70(2):119-24. View

Gopalakrishnan A, Kumar N . Antimalarial action of artesunate involves DNA damage mediated by reactive oxygen species. Antimicrob Agents Chemother. 2014; 59(1):317-25. PMC: 4291367. DOI: 10.1128/AAC.03663-14. View

McCaig C, Rajnicek A, Song B, Zhao M . Controlling cell behavior electrically: current views and future potential. Physiol Rev. 2005; 85(3):943-78. DOI: 10.1152/physrev.00020.2004. View

Zangeneh M, Mozdarani H, Mahmoudzadeh A . Potent radioprotective effects of combined regimens of famotidine and vitamin C against radiation-induced micronuclei in mouse bone marrow erythrocytes. Radiat Environ Biophys. 2015; 54(2):175-81. DOI: 10.1007/s00411-015-0586-5. View

Jakubowski W, Bartosz G . 2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure?. Cell Biol Int. 2000; 24(10):757-60. DOI: 10.1006/cbir.2000.0556. View

Tanabe K, Mikkelsen R, Wallach D . Calcium transport of Plasmodium chabaudi-infected erythrocytes. J Cell Biol. 1982; 93(3):680-4. PMC: 2112141. DOI: 10.1083/jcb.93.3.680. View

Gittens R, Olivares-Navarrete R, Tannenbaum R, Boyan B, Schwartz Z . Electrical implications of corrosion for osseointegration of titanium implants. J Dent Res. 2011; 90(12):1389-97. PMC: 3215755. DOI: 10.1177/0022034511408428. View

Golberg A, Bei M, Sheridan R, Yarmush M . Regeneration and control of human fibroblast cell density by intermittently delivered pulsed electric fields. Biotechnol Bioeng. 2013; 110(6):1759-68. DOI: 10.1002/bit.24831. View

10.

Bacarin C, de Sa-Nakanishi A, Bracht A, Matsushita M, Previdelli I, Mori M . Fish oil prevents oxidative stress and exerts sustained antiamnesic effect after global cerebral ischemia. CNS Neurol Disord Drug Targets. 2015; 14(3):400-10. DOI: 10.2174/1871527314666150225125232. View

11.

Nelson F, Brighton C, Ryaby J, Simon B, Nielson J, Lorich D . Use of physical forces in bone healing. J Am Acad Orthop Surg. 2003; 11(5):344-54. DOI: 10.5435/00124635-200309000-00007. View

12.

Draz E, Abdin A, Sarhan N, Gabr T . Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs. Pharmacol Rep. 2015; 67(2):317-25. DOI: 10.1016/j.pharep.2014.10.007. View

13.

Pak O, Sommer N, Hoeres T, Bakr A, Waisbrod S, Sydykov A . Mitochondrial hyperpolarization in pulmonary vascular remodeling. Mitochondrial uncoupling protein deficiency as disease model. Am J Respir Cell Mol Biol. 2013; 49(3):358-67. DOI: 10.1165/rcmb.2012-0361OC. View

14.

Titushkin I, Cho M . Controlling cellular biomechanics of human mesenchymal stem cells. Annu Int Conf IEEE Eng Med Biol Soc. 2009; 2009:2090-3. DOI: 10.1109/IEMBS.2009.5333949. View

15.

Zhao H, Steiger A, Nohner M, Ye H . Specific Intensity Direct Current (DC) Electric Field Improves Neural Stem Cell Migration and Enhances Differentiation towards βIII-Tubulin+ Neurons. PLoS One. 2015; 10(6):e0129625. PMC: 4466259. DOI: 10.1371/journal.pone.0129625. View

16.

Martiney J, Cerami A, Slater A . Verapamil reversal of chloroquine resistance in the malaria parasite Plasmodium falciparum is specific for resistant parasites and independent of the weak base effect. J Biol Chem. 1995; 270(38):22393-8. DOI: 10.1074/jbc.270.38.22393. View

17.

Kim J, Chu J, Shen X, Wang J, Orkin S . An extended transcriptional network for pluripotency of embryonic stem cells. Cell. 2008; 132(6):1049-61. PMC: 3837340. DOI: 10.1016/j.cell.2008.02.039. View

18.

Keitany G, Vignali M, Wang R . Live attenuated pre-erythrocytic malaria vaccines. Hum Vaccin Immunother. 2014; 10(10):2903-9. PMC: 5443077. DOI: 10.4161/21645515.2014.972764. View

19.

Gittens R, Olivares-Navarrete R, Rettew R, Butera R, Alamgir F, Boyan B . Electrical polarization of titanium surfaces for the enhancement of osteoblast differentiation. Bioelectromagnetics. 2013; 34(8):599-612. PMC: 4009505. DOI: 10.1002/bem.21810. View

20.

Haynes J, Diggs C, Hines F, Desjardins R . Culture of human malaria parasites Plasmodium falciparum. Nature. 1976; 263(5580):767-9. DOI: 10.1038/263767a0. View