Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes

Overview

Journal Curr Protoc Stem Cell Biol

Specialty Cell Biology

Date 2016 Aug 18

PMID 27532816

Citations 11

Authors

Araceli Espinosa-Jeffrey

Bruno Blanchi

Juan Carlos Biancotti

Shalini Kumar

Megumi Hirose

Berhan Mandefro

Dodanim Talavera-Adame

Nissim Benvenisty

Jean de Vellis

Affiliations

Soon will be listed here.

Abstract

Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation-our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling. © 2016 by John Wiley & Sons, Inc.

Citing Articles

Space Flight Enhances Stress Pathways in Human Neural Stem Cells.

Carpo N, Tran V, Biancotti J, Cepeda C, Espinosa-Jeffrey A Biomolecules. 2024; 14(1).

PMID: 38254665 PMC: 10813251. DOI: 10.3390/biom14010065.

Metabolomics Profile of the Secretome of Space-Flown Oligodendrocytes.

Vergnes L, Foucaud B, Cepeda C, Espinosa-Jeffrey A Cells. 2023; 12(18).

PMID: 37759473 PMC: 10528075. DOI: 10.3390/cells12182249.

Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight.

Tran V, Carpo N, Cepeda C, Espinosa-Jeffrey A Biomolecules. 2023; 13(2).

PMID: 36830573 PMC: 9953055. DOI: 10.3390/biom13020201.

Delayed Maturation of Oligodendrocyte Progenitors by Microgravity: Implications for Multiple Sclerosis and Space Flight.

Tran V, Carpo N, Shaka S, Zamudio J, Choi S, Cepeda C Life (Basel). 2022; 12(6).

PMID: 35743828 PMC: 9224676. DOI: 10.3390/life12060797.

Physiologically-Relevant Cellular Models for Biomarkers and Drug Discovery.

Jeffrey A Ann Stem Cell Res Ther. 2021; 2(2).

PMID: 34485994 PMC: 8412170.

References

Goldman S . Progenitor cell-based treatment of the pediatric myelin disorders. Arch Neurol. 2011; 68(7):848-56. PMC: 3358919. DOI: 10.1001/archneurol.2011.46. View

Redmond Jr D, Bjugstad K, Teng Y, Ourednik V, Ourednik J, Wakeman D . Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells. Proc Natl Acad Sci U S A. 2007; 104(29):12175-80. PMC: 1896134. DOI: 10.1073/pnas.0704091104. View

Sim F, Windrem M, Goldman S . Fate determination of adult human glial progenitor cells. Neuron Glia Biol. 2009; 5(3-4):45-55. DOI: 10.1017/S1740925X09990317. View

Espinosa-Jeffrey A, Wakeman D, Kim S, Snyder E, de Vellis J . Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation. Curr Protoc Stem Cell Biol. 2009; Chapter 2:Unit 2D.4. DOI: 10.1002/9780470151808.sc02d04s10. View

Espinosa de Los Monteros A, Yuan J, McCartney D, Madrid R, Cole R, Kanfer J . Acceleration of the maturation of oligodendroblasts into oligodendrocytes and enhancement of their myelinogenic properties by a chemically defined medium. Dev Neurosci. 1997; 19(4):297-311. DOI: 10.1159/000111226. View

Mauro V, Wood I, Krushel L, Crossin K, Edelman G . Cell adhesion alters gene transcription in chicken embryo brain cells and mouse embryonal carcinoma cells. Proc Natl Acad Sci U S A. 1994; 91(7):2868-72. PMC: 43472. DOI: 10.1073/pnas.91.7.2868. View

Yan J, Welsh A, Bora S, Snyder E, Koliatsos V . Differentiation and tropic/trophic effects of exogenous neural precursors in the adult spinal cord. J Comp Neurol. 2004; 480(1):101-14. DOI: 10.1002/cne.20344. View

Chattopadhyay N, Espinosa-Jeffrey A, Tfelt-Hansen J, Yano S, Bandyopadhyay S, Brown E . Calcium receptor expression and function in oligodendrocyte commitment and lineage progression: potential impact on reduced myelin basic protein in CaR-null mice. J Neurosci Res. 2008; 86(10):2159-67. DOI: 10.1002/jnr.21662. View

Linnemann D, Bock E . Cell adhesion molecules in neural development. Dev Neurosci. 1989; 11(3):149-73. DOI: 10.1159/000111896. View

10.

Zhang S, Lundberg C, Lipsitz D, OConnor L, Duncan I . Generation of oligodendroglial progenitors from neural stem cells. J Neurocytol. 2001; 27(7):475-89. DOI: 10.1023/a:1006953023845. View

11.

Espinosa de Los Monteros A, Bernard R, Tiller B, Rouget P, de Vellis J . Grafting of fast blue labeled glial cells into neonatal rat brain: differential survival and migration among cell types. Int J Dev Neurosci. 1993; 11(5):625-39. DOI: 10.1016/0736-5748(93)90051-e. View

12.

Snyder E, Deitcher D, Walsh C, Hartwieg E, Cepko C . Multipotent neural cell lines can engraft and participate in development of mouse cerebellum. Cell. 1992; 68(1):33-51. DOI: 10.1016/0092-8674(92)90204-p. View

13.

Li B, Carey M, Workman J . The role of chromatin during transcription. Cell. 2007; 128(4):707-19. DOI: 10.1016/j.cell.2007.01.015. View

14.

Mo Z, Zecevic N . Human fetal radial glia cells generate oligodendrocytes in vitro. Glia. 2008; 57(5):490-8. PMC: 2644732. DOI: 10.1002/glia.20775. View

15.

Flax J, Aurora S, Yang C, Simonin C, Wills A, Billinghurst L . Engraftable human neural stem cells respond to developmental cues, replace neurons, and express foreign genes. Nat Biotechnol. 1998; 16(11):1033-9. DOI: 10.1038/3473. View

16.

Saneto R, de Vellis J . Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium. Proc Natl Acad Sci U S A. 1985; 82(10):3509-13. PMC: 397806. DOI: 10.1073/pnas.82.10.3509. View

17.

Snyder E, Yoon C, Flax J, Macklis J . Multipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex. Proc Natl Acad Sci U S A. 1997; 94(21):11663-8. PMC: 23575. DOI: 10.1073/pnas.94.21.11663. View

18.

Espinosa de Los Monteros A, Zhang M, Gordon M, Aymie M, de Vellis J . Transplantation of cultured premyelinating oligodendrocytes into normal and myelin-deficient rat brain. Dev Neurosci. 1992; 14(2):98-104. DOI: 10.1159/000111653. View

19.

Espinosa de Los Monteros A, Zhang M, de Vellis J . O2A progenitor cells transplanted into the neonatal rat brain develop into oligodendrocytes but not astrocytes. Proc Natl Acad Sci U S A. 1993; 90(1):50-4. PMC: 45597. DOI: 10.1073/pnas.90.1.50. View

20.

Yandava B, Billinghurst L, Snyder E . "Global" cell replacement is feasible via neural stem cell transplantation: evidence from the dysmyelinated shiverer mouse brain. Proc Natl Acad Sci U S A. 1999; 96(12):7029-34. PMC: 22044. DOI: 10.1073/pnas.96.12.7029. View