Experimental Primers Containing Synthetic and Natural Compounds Reduce Enzymatic Activity at the Dentin-adhesive Interface Under Cyclic Loading

Overview

Journal Dent Mater

Specialty Dentistry

Date 2016 Aug 16

PMID 27524231

Citations 13

Authors

Ana Beatriz Silva Sousa

Cristina M P Vidal

Ariene Arcas Leme-Kraus

Fernanda C P Pires-de-Souza

Ana K Bedran-Russo

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the effect of experimental primers (chlorhexidine, enriched mixture of proanthocyanidins, and doxycycline) on the adhesive properties and gelatinolytic activity at dentin-resin interfaces of occlusal Class I restorations.

Methods: The inactivation of enzymes by the experimental primers was assessed by fluorescence assay and gelatin zymography. To assess the adhesive properties, occlusal Class I cavities were prepared in sound human molars, etched with phosphoric acid and restored with one of the primers and an etch-and-rinse adhesive system (Adper Single Bond Plus-3M ESPE). After the restorative procedures, specimens were divided into two subgroups (n=6) consisting of storage in incubation buffer or axial cyclic loading at 50N and 1,000,000 cycles. Then, the specimens were sectioned and slices were assigned to in situ zymography assay and microtensile bond strength (TBS) test.

Results: Fluorescence assay and gelatin zymography revealed that the experimental primers inactivated rMMPs. In situ zymography (2-way ANOVA, Tukey, p<0.05) showed that cyclic loading increased the gelatinolytic activity at the resin-dentin interface and the experimental primers decreased the gelatinolytic activity at the adhesive interface. The experimental primers had no significant effects on dentin-adhesive bond strengths with or without cyclic loading (2-way ANOVA, p>0.05).

Significance: The use of experimental primers impaired the enzymatic activity at the dentin-adhesive interface after cyclic loading and the activity of rMMPs. Cyclic loading did not have a significant effect on the bond strength.

Citing Articles

Fucosterol and Fucoxanthin Enhance Dentin Collagen Stability and Erosion Resistance Through Crosslinking and MMP Inhibition.

Lee W, Kim Y, Bae M, Yoo K, Park H, Kim Y Int J Nanomedicine. 2024; 19:13253-13265.

PMID: 39679254 PMC: 11639964. DOI: 10.2147/IJN.S490667.

Exploring the effectiveness of doxycycline in restorative dentistry: a systematic review of in vitro studies.

Carneiro B, Prado M, Nogueira I, Moreira A, Andre C Odontology. 2024; 113(1):15-41.

PMID: 39133374 DOI: 10.1007/s10266-024-00986-2.

Effects of proanthocyanidin-functionalized hydroxyapatite nanoparticles on dentin bonding.

Enrich-Essvein T, Gonzalez-Lopez S, Rodriguez-Navarro A, Cifuentes-Jimenez C, Maravic T, Mazzitelli C Clin Oral Investig. 2024; 28(8):444.

PMID: 39046575 PMC: 11269515. DOI: 10.1007/s00784-024-05836-7.

Effect of the use of bromelain associated with bioactive glass-ceramic on dentin/adhesive interface.

Geng Vivanco R, Sousa A, Oliveira V, Sinhoreti M, de Carvalho Panzeri Pires-de-Souza F Clin Oral Investig. 2024; 28(1):106.

PMID: 38244108 DOI: 10.1007/s00784-024-05496-7.

Development of Proanthocyanidin-Loaded Mesoporous Silica Nanoparticles for Improving Dental Adhesion.

Alkhazaleh A, Elfagih S, Chakka L, Armstrong S, Comnick C, Qian F Mol Pharm. 2022; 19(12):4675-4684.

PMID: 36349888 PMC: 9727728. DOI: 10.1021/acs.molpharmaceut.2c00728.

References

Stanislawczuk R, Pereira F, Munoz M, Luque I, Farago P, Reis A . Effects of chlorhexidine-containing adhesives on the durability of resin-dentine interfaces. J Dent. 2013; 42(1):39-47. DOI: 10.1016/j.jdent.2013.11.002. View

Xu X, Chen Z, Wang Y, Yamada Y, Steffensen B . Functional basis for the overlap in ligand interactions and substrate specificities of matrix metalloproteinases-9 and -2. Biochem J. 2005; 392(Pt 1):127-34. PMC: 1317671. DOI: 10.1042/BJ20050650. View

Liu Y, Tjaderhane L, Breschi L, Mazzoni A, Li N, Mao J . Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res. 2011; 90(8):953-68. PMC: 3148178. DOI: 10.1177/0022034510391799. View

Scheffel D, Delgado C, Soares D, Basso F, de Souza Costa C, Pashley D . Increased Durability of Resin-Dentin Bonds Following Cross-Linking Treatment. Oper Dent. 2015; 40(5):533-9. PMC: 5657488. DOI: 10.2341/13-211-L. View

Seseogullari-Dirihan R, Mutluay M, Vallittu P, Pashley D, Tezvergil-Mutluay A . Effect of pretreatment with collagen crosslinkers on dentin protease activity. Dent Mater. 2015; 31(8):941-7. PMC: 4617191. DOI: 10.1016/j.dental.2015.05.002. View

Sulkala M, Wahlgren J, Larmas M, Sorsa T, Teronen O, Salo T . The effects of MMP inhibitors on human salivary MMP activity and caries progression in rats. J Dent Res. 2001; 80(6):1545-9. DOI: 10.1177/00220345010800061301. View

Newton A, Cadena S, Rocha M, Carnieri E, de Oliveira M . New data on biological effects of chlorhexidine: Fe2+ induced lipid peroxidation and mitochondrial permeability transition. Toxicol Lett. 2004; 151(3):407-16. DOI: 10.1016/j.toxlet.2004.02.013. View

Cecchin D, Pin L, Farina A, Souza M, Vidal C, Bello Y . Bond Strength between Fiber Posts and Root Dentin Treated with Natural Cross-linkers. J Endod. 2015; 41(10):1667-71. DOI: 10.1016/j.joen.2015.05.022. View

Mobarak E . Effect of chlorhexidine pretreatment on bond strength durability of caries-affected dentin over 2-year aging in artificial saliva and under simulated intrapulpal pressure. Oper Dent. 2011; 36(6):649-60. DOI: 10.2341/11-018-L. View

10.

Kudalkar M, Nayak A, Bhat K, Nayak R . Effect of Azadirachta indica (Neem) and Aloe vera as compared to subantimicrobial dose doxycycline on matrix metalloproteinases (MMP)-2 and MMP-9: An in-vitro study. Ayu. 2014; 35(1):85-9. PMC: 4213975. DOI: 10.4103/0974-8520.141947. View

11.

Feitosa S, Palasuk J, Kamocki K, Geraldeli S, Gregory R, Platt J . Doxycycline-encapsulated nanotube-modified dentin adhesives. J Dent Res. 2014; 93(12):1270-6. PMC: 4462804. DOI: 10.1177/0022034514549997. View

12.

Tjaderhane L, Nascimento F, Breschi L, Mazzoni A, Tersariol I, Geraldeli S . Strategies to prevent hydrolytic degradation of the hybrid layer-A review. Dent Mater. 2013; 29(10):999-1011. PMC: 3899917. DOI: 10.1016/j.dental.2013.07.016. View

13.

Castellan C, Bedran-Russo A, Antunes A, Pereira P . Effect of dentin biomodification using naturally derived collagen cross-linkers: one-year bond strength study. Int J Dent. 2013; 2013:918010. PMC: 3771447. DOI: 10.1155/2013/918010. View

14.

Phansalkar R, Nam J, Chen S, McAlpine J, Napolitano J, Leme A . A galloylated dimeric proanthocyanidin from grape seed exhibits dentin biomodification potential. Fitoterapia. 2014; 101:169-78. PMC: 4346468. DOI: 10.1016/j.fitote.2014.12.006. View

15.

Iskander M, Elkassas D, Mohsen M . Effect of two matrix metalloproteinase inhibitors on the color stability of a nanofilled resin composite. Oper Dent. 2014; 40(1):E11-20. DOI: 10.2341/12-428-L. View

16.

Zheng P, Zaruba M, Attin T, Wiegand A . Effect of different matrix metalloproteinase inhibitors on microtensile bond strength of an etch-and-rinse and a self-etching adhesive to dentin. Oper Dent. 2014; 40(1):80-6. DOI: 10.2341/13-162-L. View

17.

Carrilho M, Carvalho R, Sousa E, Nicolau J, Breschi L, Mazzoni A . Substantivity of chlorhexidine to human dentin. Dent Mater. 2010; 26(8):779-85. PMC: 2909541. DOI: 10.1016/j.dental.2010.04.002. View

18.

Hebling J, Pashley D, Tjaderhane L, Tay F . Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res. 2005; 84(8):741-6. DOI: 10.1177/154405910508400811. View

19.

De Munck J, Van den Steen P, Mine A, Van Landuyt K, Poitevin A, Opdenakker G . Inhibition of enzymatic degradation of adhesive-dentin interfaces. J Dent Res. 2009; 88(12):1101-6. DOI: 10.1177/0022034509346952. View

20.

Tay F, Pashley D, Loushine R, Weller R, Monticelli F, Osorio R . Self-etching adhesives increase collagenolytic activity in radicular dentin. J Endod. 2006; 32(9):862-8. DOI: 10.1016/j.joen.2006.04.005. View