Real-time Imaging and Quantitative Analysis of Doxorubicin Transport in a Perfusable Microvessel Platform

Overview

Journal Integr Biol (Camb)

Publisher Oxford University Press

Specialty Biology

Date 2016 Aug 16

PMID 27523481

Citations 11

Authors

Max I Bogorad

Peter C Searson

Affiliations

Soon will be listed here.

Abstract

Here we report on real-time imaging and quantitative analysis of solute transport in perfusable cylindrical microvessels formed from Madin-Darby canine kidney (MDCK) cells embedded in a collagen matrix. Fluorescence microscopy was used to image the kinetics of doxorubicin transport following injection. To assess the role of efflux pumps on transport, experiments were performed in microvessels formed from MDCK.2, MDCKII-w/t, and MDCKII-MDR1 cells. MDCKII-w/t and MDCKII-MDR1 showed significant doxorubicin accumulation in the cells, characteristic of the pharmacokinetics of doxorubicin. We present a model for doxorubicin transport that takes into account transport across the cell layer. These results demonstrate how real-time imaging of cell microvessels can be used to analyze the mechanisms of transport and distribution following systemic delivery.

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