In Vitro Studies on Interaction of Rat Pancreatic Lipase and Colipase with Biliary Lipids

Lipase and colipase were prepared separately from rat pancreatic juice, and their respective interaction with biliary lipids was investigated by gel filtration on agarose in the presence of a micellar solution of sodium taurocholate. It was found that the cofactor can associate with the biliary lipids, whereas the enzyme forms a high mol wt complex only in the presence of colipase. It is suggested that biliary phospholipids might participate in the in vivo formation of the enzyme-cofactor substrate complex at the triglyceride-water interface in the presence of bile salts.