HIRA Is Required for Heart Development and Directly Regulates Tnni2 and Tnnt3

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Aug 13

PMID 27518902

Citations 16

Authors

Daniel Dilg

Rasha Noureldin M Saleh

Sarah Elizabeth Lee Phelps

Yoann Rose

Laurent Dupays

Cian Murphy

Timothy Mohun

Robert H Anderson

Peter J Scambler

Ariane L A Chapgier

Affiliations

Soon will be listed here.

Abstract

Chromatin remodelling is essential for cardiac development. Interestingly, the role of histone chaperones has not been investigated in this regard. HIRA is a member of the HUCA (HIRA/UBN1/CABIN1/ASF1a) complex that deposits the variant histone H3.3 on chromatin independently of replication. Lack of HIRA has general effects on chromatin and gene expression dynamics in embryonic stem cells and mouse oocytes. Here we describe the conditional ablation of Hira in the cardiogenic mesoderm of mice. We observed surface oedema, ventricular and atrial septal defects and embryonic lethality. We identified dysregulation of a subset of cardiac genes, notably upregulation of troponins Tnni2 and Tnnt3, involved in cardiac contractility and decreased expression of Epha3, a gene necessary for the fusion of the muscular ventricular septum and the atrioventricular cushions. We found that HIRA binds GAGA rich DNA loci in the embryonic heart, and in particular a previously described enhancer of Tnni2/Tnnt3 (TTe) bound by the transcription factor NKX2.5. HIRA-dependent H3.3 enrichment was observed at the TTe in embryonic stem cells (ESC) differentiated toward cardiomyocytes in vitro. Thus, we show here that HIRA has locus-specific effects on gene expression and that histone chaperone activity is vital for normal heart development, impinging on pathways regulated by an established cardiac transcription factor.

Citing Articles

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HIRA vs. DAXX: the two axes shaping the histone H3.3 landscape.

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