Bacillus Anthracis S-layer Protein BslA Binds to Extracellular Matrix by Interacting with Laminin

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2016 Aug 13

PMID 27514510

Citations 7

Authors

Yanchun Wang

Ying Wei

Shengling Yuan

Haoxia Tao

Jie Dong

Zhaoshan Zhang

Wei Tian

Chunjie Liu

Affiliations

Soon will be listed here.

Abstract

Background: The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to identify the potential eukaryotic ligand(s) for B. anthracis BslA protein.

Results: Biochemical approaches have indicated that the putative host cell ligand(s) for BslA is a surface protein, which is independent of the sugar components for binding to Bs1A. A ligand screening using blot overlays, far Western blots and mass spectrometry analyses revealed that BslA binds to mammalian laminin. ELISA based solid-phase binding assays and surface plasmon resonance assays demonstrated that there were high affinity interactions between BslA(260-652) and laminin. The SPR results also revealed the dissociation constants values of 3.172 × 10(-9)M for the binding of BslA(260-652) to laminin.

Conclusions: These data demonstrated that laminin is a ligand for BslA.

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References

Agarwal S, Kulshreshtha P, Bambah Mukku D, Bhatnagar R . alpha-Enolase binds to human plasminogen on the surface of Bacillus anthracis. Biochim Biophys Acta. 2008; 1784(7-8):986-94. DOI: 10.1016/j.bbapap.2008.03.017. View

Heilmann C . Adhesion mechanisms of staphylococci. Adv Exp Med Biol. 2011; 715:105-23. DOI: 10.1007/978-94-007-0940-9_7. View

Steichen C, Kearney J, Turnbough Jr C . Non-uniform assembly of the Bacillus anthracis exosporium and a bottle cap model for spore germination and outgrowth. Mol Microbiol. 2007; 64(2):359-67. DOI: 10.1111/j.1365-2958.2007.05658.x. View

Pizarro-Cerda J, Cossart P . Bacterial adhesion and entry into host cells. Cell. 2006; 124(4):715-27. DOI: 10.1016/j.cell.2006.02.012. View

Otto M . Targeted immunotherapy for staphylococcal infections : focus on anti-MSCRAMM antibodies. BioDrugs. 2008; 22(1):27-36. DOI: 10.2165/00063030-200822010-00003. View

Cozens D, Read R . Anti-adhesion methods as novel therapeutics for bacterial infections. Expert Rev Anti Infect Ther. 2012; 10(12):1457-68. DOI: 10.1586/eri.12.145. View

Tenenbaum T, Spellerberg B, Adam R, Vogel M, Kim K, Schroten H . Streptococcus agalactiae invasion of human brain microvascular endothelial cells is promoted by the laminin-binding protein Lmb. Microbes Infect. 2007; 9(6):714-20. DOI: 10.1016/j.micinf.2007.02.015. View

Combet C, Blanchet C, Geourjon C, Deleage G . NPS@: network protein sequence analysis. Trends Biochem Sci. 2000; 25(3):147-50. DOI: 10.1016/s0968-0004(99)01540-6. View

Patti J, Allen B, McGavin M, Hook M . MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol. 1994; 48:585-617. DOI: 10.1146/annurev.mi.48.100194.003101. View

10.

Koehler T . Bacillus anthracis physiology and genetics. Mol Aspects Med. 2009; 30(6):386-96. PMC: 2784286. DOI: 10.1016/j.mam.2009.07.004. View

11.

Shatalin K, Neyfakh A . Efficient gene inactivation in Bacillus anthracis. FEMS Microbiol Lett. 2005; 245(2):315-9. DOI: 10.1016/j.femsle.2005.03.029. View

12.

Kline K, Falker S, Dahlberg S, Normark S, Henriques-Normark B . Bacterial adhesins in host-microbe interactions. Cell Host Microbe. 2009; 5(6):580-92. DOI: 10.1016/j.chom.2009.05.011. View

13.

Brahmbhatt T, Janes B, Stibitz E, Darnell S, Sanz P, Rasmussen S . Bacillus anthracis exosporium protein BclA affects spore germination, interaction with extracellular matrix proteins, and hydrophobicity. Infect Immun. 2007; 75(11):5233-9. PMC: 2168272. DOI: 10.1128/IAI.00660-07. View

14.

Wang X, Ge J, Liu B, Hu Y, Yang M . Structures of SdrD from Staphylococcus aureus reveal the molecular mechanism of how the cell surface receptors recognize their ligands. Protein Cell. 2013; 4(4):277-85. PMC: 4875524. DOI: 10.1007/s13238-013-3009-x. View

15.

Kern J, Schneewind O . BslA, a pXO1-encoded adhesin of Bacillus anthracis. Mol Microbiol. 2008; 68(2):504-15. DOI: 10.1111/j.1365-2958.2008.06169.x. View

16.

Hartmann M, Papavlassopoulos H, Chandrasekaran V, Grabosch C, Beiroth F, Lindhorst T . Inhibition of bacterial adhesion to live human cells: activity and cytotoxicity of synthetic mannosides. FEBS Lett. 2012; 586(10):1459-65. DOI: 10.1016/j.febslet.2012.03.059. View

17.

Louis-Jeune C, Andrade-Navarro M, Perez-Iratxeta C . Prediction of protein secondary structure from circular dichroism using theoretically derived spectra. Proteins. 2011; 80(2):374-81. DOI: 10.1002/prot.23188. View

18.

Chung M, Jorgensen S, Tonry J, Kashanchi F, Bailey C, Popov S . Secreted Bacillus anthracis proteases target the host fibrinolytic system. FEMS Immunol Med Microbiol. 2011; 62(2):173-81. DOI: 10.1111/j.1574-695X.2011.00798.x. View

19.

Barbu E, Ganesh V, Gurusiddappa S, Mackenzie R, Foster T, Sudhof T . beta-Neurexin is a ligand for the Staphylococcus aureus MSCRAMM SdrC. PLoS Pathog. 2010; 6(1):e1000726. PMC: 2800189. DOI: 10.1371/journal.ppat.1000726. View

20.

Ragunathan P, Spellerberg B, Ponnuraj K . Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae. Acta Crystallogr D Biol Crystallogr. 2009; 65(Pt 12):1262-9. DOI: 10.1107/S0907444909038359. View