Design and Cellular Studies of a Carbon Nanotube-based Delivery System for a Hybrid Platinum-acridine Anticancer Agent

Overview

Journal J Inorg Biochem

Specialty Biochemistry

Date 2016 Aug 7

PMID 27496614

Citations 3

Authors

Cale D Fahrenholtz

Song Ding

Brian W Bernish

Mariah L Wright

Ye Zheng

Mu Yang

Xiyuan Yao

George L Donati

Michael D Gross

Ulrich Bierbach

Ravi Singh

Affiliations

Soon will be listed here.

Abstract

A three-component drug-delivery system has been developed consisting of multi-walled carbon nanotubes (MWCNTs) coated with a non-classical platinum chemotherapeutic agent ([PtCl(NH)(L)]Cl (P3A1; L=N-(2-(acridin-9-ylamino)ethyl)-N-methylproprionimidamide) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (DSPE-mPEG). The optimized P3A1-MWCNTs are colloidally stable in physiological solution and deliver more P3A1 into breast cancer cells than treatment with the free drug. Furthermore, P3A1-MWCNTs are cytotoxic to several cell models of breast cancer and induce S-phase cell cycle arrest and non-apoptotic cell death in breast cancer cells. By contrast, free P3A1 induces apoptosis and allows progression to G2/M phase. Photothermal activation of P3A1-MWCNTs to generate mild hyperthermia potentiates their cytotoxicity. These findings suggest that delivery of P3A1 to cancer cells using MWCNTs as a drug carrier may be beneficial for combination cancer chemotherapy and photothermal therapy.

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