Reconstitution of Intracellular Environments in Vitro and in Artificial Cells

Overview

Journal Biophysics (Nagoya-shi)

Specialty Biophysics

Date 2016 Aug 6

PMID 27493497

Citations 6

Authors

Kei Fujiwara

Miho Yanagisawa

Shin-Ichiro M Nomura

Affiliations

Soon will be listed here.

Abstract

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.

Citing Articles

Cell-size space effects on phase separation of binary polymer blends.

Yanagisawa M Biophys Rev. 2022; 14(5):1093-1103.

PMID: 36345284 PMC: 9636348. DOI: 10.1007/s12551-022-01001-0.

Thermo-Statistical Effects of Inclusions on Vesicles: Division into Multispheres and Polyhedral Deformation.

Natsume Y Membranes (Basel). 2022; 12(6).

PMID: 35736315 PMC: 9229943. DOI: 10.3390/membranes12060608.

System concentration shift as a regulator of transcription-translation system within liposomes.

Akui T, Fujiwara K, Sato G, Takinoue M, Nomura S, Doi N iScience. 2021; 24(8):102859.

PMID: 34386726 PMC: 8346668. DOI: 10.1016/j.isci.2021.102859.

A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression.

Takahashi K, Sato G, Doi N, Fujiwara K Life (Basel). 2021; 11(3).

PMID: 33805612 PMC: 7999496. DOI: 10.3390/life11030237.

In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile.

Fujiwara K, Sawamura T, Niwa T, Deyama T, Nomura S, Taguchi H Nucleic Acids Res. 2017; 45(19):11449-11458.

PMID: 28977538 PMC: 5737407. DOI: 10.1093/nar/gkx776.

References

Kuruma Y, Stano P, Ueda T, Luisi P . A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells. Biochim Biophys Acta. 2008; 1788(2):567-74. DOI: 10.1016/j.bbamem.2008.10.017. View

Caschera F, Noireaux V . Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system. Biochimie. 2013; 99:162-8. DOI: 10.1016/j.biochi.2013.11.025. View

Nomura S, Yoshikawa Y, Yoshikawa K, Dannenmuller O, Chasserot-Golaz S, Ourisson G . Towards proto-cells: "primitive" lipid vesicles encapsulating giant DNA and its histone complex. Chembiochem. 2002; 2(6):457-9. DOI: 10.1002/1439-7633(20010601)2:6<457::AID-CBIC457>3.0.CO;2-F. View

Fuller R, Kaguni J, Kornberg A . Enzymatic replication of the origin of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1981; 78(12):7370-4. PMC: 349268. DOI: 10.1073/pnas.78.12.7370. View

Tanaka-Takiguchi Y, Itoh T, Tsujita K, Yamada S, Yanagisawa M, Fujiwara K . Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual F-BAR domain proteins. Langmuir. 2012; 29(1):328-36. DOI: 10.1021/la303902q. View

Tsuji A, Yoshikawa K . ON-OFF switching of transcriptional activity of large DNA through a conformational transition in cooperation with phospholipid membrane. J Am Chem Soc. 2010; 132(35):12464-71. PMC: 2931404. DOI: 10.1021/ja105154k. View

Noireaux V, Libchaber A . A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A. 2004; 101(51):17669-74. PMC: 539773. DOI: 10.1073/pnas.0408236101. View

Albertsson P, Cajarville A, Brooks D, Tjerneld F . Partition of proteins in aqueous polymer two-phase systems and the effect of molecular weight of the polymer. Biochim Biophys Acta. 1987; 926(1):87-93. DOI: 10.1016/0304-4165(87)90185-1. View

Osawa M, Anderson D, Erickson H . Reconstitution of contractile FtsZ rings in liposomes. Science. 2008; 320(5877):792-4. PMC: 2645864. DOI: 10.1126/science.1154520. View

10.

Shin J, Noireaux V . An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells. ACS Synth Biol. 2013; 1(1):29-41. DOI: 10.1021/sb200016s. View

11.

Fujiwara K, Yanagisawa M . Generation of giant unilamellar liposomes containing biomacromolecules at physiological intracellular concentrations using hypertonic conditions. ACS Synth Biol. 2014; 3(12):870-4. DOI: 10.1021/sb4001917. View

12.

Fujii S, Matsuura T, Sunami T, Kazuta Y, Yomo T . In vitro evolution of α-hemolysin using a liposome display. Proc Natl Acad Sci U S A. 2013; 110(42):16796-801. PMC: 3801045. DOI: 10.1073/pnas.1314585110. View

13.

Yanagisawa M, Imai M, Taniguchi T . Shape deformation of ternary vesicles coupled with phase separation. Phys Rev Lett. 2008; 100(14):148102. DOI: 10.1103/PhysRevLett.100.148102. View

14.

Zimmerman S, Trach S . Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli. J Mol Biol. 1991; 222(3):599-620. DOI: 10.1016/0022-2836(91)90499-v. View

15.

Minton A . How can biochemical reactions within cells differ from those in test tubes?. J Cell Sci. 2006; 119(Pt 14):2863-9. DOI: 10.1242/jcs.03063. View

16.

Nomura S, Tsumoto K, Hamada T, Akiyoshi K, Nakatani Y, Yoshikawa K . Gene expression within cell-sized lipid vesicles. Chembiochem. 2003; 4(11):1172-5. DOI: 10.1002/cbic.200300630. View

17.

Parry B, Surovtsev I, Cabeen M, OHern C, Dufresne E, Jacobs-Wagner C . The bacterial cytoplasm has glass-like properties and is fluidized by metabolic activity. Cell. 2013; 156(1-2):183-94. PMC: 3956598. DOI: 10.1016/j.cell.2013.11.028. View

18.

Pautot S, Frisken B, Weitz D . Engineering asymmetric vesicles. Proc Natl Acad Sci U S A. 2003; 100(19):10718-21. PMC: 196870. DOI: 10.1073/pnas.1931005100. View

19.

Yu W, Sato K, Wakabayashi M, Nakaishi T, Ko-Mitamura E, Shima Y . Synthesis of functional protein in liposome. J Biosci Bioeng. 2005; 92(6):590-3. DOI: 10.1263/jbb.92.590. View

20.

Doolittle W . Uprooting the tree of life. Sci Am. 2000; 282(2):90-5. DOI: 10.1038/scientificamerican0200-90. View