Depletion of Dicer Promotes Epithelial Ovarian Cancer Progression by Elevating PDIA3 Expression

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2016 Aug 6

PMID 27492604

Citations 9

Authors

Ying Zhu

Liqiong Cai

Jing Guo

Na Chen

Xiaoqing Yi

Yong Zhao

Jing Cai

Zehua Wang

Affiliations

Soon will be listed here.

Abstract

Dicer is an essential component of the microRNA (miRNA) processing machinery whose low expression is associated with advanced stage and poor clinical outcome in epithelial ovarian cancer. To investigate the functional relevance of Dicer in epithelial ovarian cancer and to identify its downstream effectors, two-dimensional gel electrophoresis combined with mass spectrometry was used for proteomic profiling. Dicer depletion promoted ovarian cancer cell proliferation and migration accompanied by a global upregulation of proteins. Twenty-six proteins, 7 upregulated and 19 downregulated, were identified. The functions of the identified proteins and their interactions were bioinformatically analyzed. Among them, protein disulfide-isomerase A3 (PDIA3) was considered to be a potential target protein of Dicer. PDIA3 repression by siRNA could significantly relieve the proliferation- and migration-promoting effect mediated by Dicer depletion in vitro and in vivo. Moreover, the miRNAs targeting PDIA3 were decreased in cells with Dicer depletion. In summary, low Dicer expression contributes to epithelial ovarian cancer progression by elevating PDIA3 expression.

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