Quantitative Proteomic Analysis for Novel Biomarkers of Buccal Squamous Cell Carcinoma Arising in Background of Oral Submucous Fibrosis

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2016 Aug 4

PMID 27485544

Citations 8

Authors

Wen Liu

Lijuan Zeng

Ning Li

Fei Wang

Canhua Jiang

Feng Guo

Xinqun Chen

Tong Su

Chunjiao Xu

Shanshan Zhang

Changyun Fang

Affiliations

Soon will be listed here.

Abstract

Background: In South and Southeast Asian, the majority of buccal squamous cell carcinoma (BSCC) can arise from oral submucous fibrosis (OSF). BSCCs develop in OSF that are often not completely resected, causing local relapse. The aim of our study was to find candidate protein biomarkers to detect OSF and predict prognosis in BSCCs by quantitative proteomics approaches.

Methods: We compared normal oral mucosa (NBM) and paired biopsies of BSCC and OSF by quantitative proteomics using isobaric tags for relative and absolute quantification (iTRAQ) to discover proteins with differential expression. Gene Ontology and KEGG networks were analyzed. The prognostic value of biomarkers was evaluated in 94 BSCCs accompanied with OSF. Significant associations were assessed by Kaplan-Meier survival and Cox-proportional hazards analysis.

Results: In total 30 proteins were identified with significantly different expression (false discovery rate < 0.05) among three tissues. Two consistently upregulated proteins, ANXA4 and FLNA, were validated. The disease-free survival was negatively associated with the expression of ANXA4 (hazard ratio, 3.4; P = 0.000), FLNA (hazard ratio, 2.1; P = 0.000) and their combination (hazard ratio, 8.8; P = 0.002) in BSCCs.

Conclusion: The present study indicates that iTRAQ quantitative proteomics analysis for tissues of BSCC and OSF is a reliable strategy. A significantly up-regulated ANXA4 and FLNA could be not only candidate biomarkers for BSCC prognosis but also potential targets for its therapy.

Citing Articles

Recent advances in minimally invasive biomarkers of OSCC: from generalized to personalized approach.

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Mechanisms and markers of malignant transformation of oral submucous fibrosis.

Lin F, Xiao T, Wang B, Wang L, Liu G, Wang R Heliyon. 2024; 10(1):e23314.

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IRF4 as a novel target involved in malignant transformation of oral submucous fibrosis into oral squamous cell carcinoma.

Meng L, Jiang Y, You J, Zhao P, Liu W, Zhao N Sci Rep. 2023; 13(1):2775.

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Pathobiological functions and clinical implications of annexin dysregulation in human cancers.

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Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis-An Indian Study.

Venugopal D, Ravindran S, Shyamsundar V, Sankarapandian S, Krishnamurthy A, Sivagnanam A J Pers Med. 2022; 12(2).

PMID: 35207696 PMC: 8878868. DOI: 10.3390/jpm12020208.

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