Contribution of DNA Polymerase Delta to DNA Replication in Permeable CHO Cells Synchronized in S Phase

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Jun 26

PMID 2748336

Citations 7

Authors

A Basnakian

G Banfalvi

N Sarkar

Affiliations

Soon will be listed here.

Abstract

To evaluate the relative contributions of DNA polymerase alpha and DNA polymerase delta in chromosome replication during the S phase of the cell cycle, we have used the permeable cell system for replication as a functional assay. We carried out the analysis of DNA polymerases both in quiescent cells stimulated to proliferate and progress through the cell cycle (monolayers) and in actively growing cells separated into progressive stages of the cell cycle by centrifugal elutriation (suspension cultures). DNA polymerase alpha was measured by using the inhibitor butylphenyl dGTP at low concentrations. Using several inhibitors such as aphidicolin, ddTTP and butylphenyl dGTP, we found that DNA polymerase alpha and delta activity were coordinately increased during S phase and declined at the end. However, DNA polymerase delta was performing about 80% of the total replication and DNA polymerase alpha performed only 20%. This high ratio of DNA polymerase delta to DNA polymerase alpha replication activity was maintained throughout S phase in two entirely different experimental approaches.

Citing Articles

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Immunofluorescent visualization of DNA replication sites within nuclei of Chinese hamster ovary cells.

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Does butylphenyl-deoxyguanosine triphosphate differentially inhibit DNA polymerase alpha and delta activities in permeabilized HeLa cells?.

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Early replication signals in nuclei of Chinese hamster ovary cells.

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Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40.

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