Spectral Phasor Analysis of LAURDAN Fluorescence in Live A549 Lung Cells to Study the Hydration and Time Evolution of Intracellular Lamellar Body-like Structures

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2016 Aug 3

PMID 27480804

Citations 37

Authors

Leonel Malacrida

Soledad Astrada

Arturo Briva

Mariela Bollati-Fogolin

Enrico Gratton

Luis A Bagatolli

Affiliations

Soon will be listed here.

Abstract

Using LAURDAN spectral imaging and spectral phasor analysis we concurrently studied the growth and hydration state of subcellular organelles (lamellar body-like, LB-like) from live A549 lung cancer cells at different post-confluence days. Our results reveal a time dependent two-step process governing the size and hydration of these intracellular LB-like structures. Specifically, a first step (days 1 to 7) is characterized by an increase in their size, followed by a second one (days 7 to 14) where the organelles display a decrease in their global hydration properties. Interestingly, our results also show that their hydration properties significantly differ from those observed in well-characterized artificial lamellar model membranes, challenging the notion that a pure lamellar membrane organization is present in these organelles at intracellular conditions. Finally, these LB-like structures show a significant increase in their hydration state upon secretion, suggesting a relevant role of entropy during this process.

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