A Long Noncoding RNA Regulates Sister Chromatid Cohesion

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2016 Aug 2

PMID 27477908

Citations 47

Authors

Francesco P Marchese

Elena Grossi

Oskar Marin-Bejar

Sanjay Kumar Bharti

Ivan Raimondi

Jovanna Gonzalez

Dannys Jorge Martinez-Herrera

Alejandro Athie

Alicia Amadoz

Robert M Brosh Jr

Maite Huarte

Affiliations

Soon will be listed here.

Abstract

Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication and sister chromatid cohesion. These findings unveil a direct role for an lncRNA in the establishment of sister chromatid cohesion by modulating DDX11 enzymatic activity.

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