Ascorbate-Deficient Vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth

Overview

Journal Front Plant Sci

Date 2016 Jul 29

PMID 27468291

Citations 25

Authors

Benson Lim

Nicholas Smirnoff

Christopher S Cobbett

John F Golz

Affiliations

Soon will be listed here.

Abstract

In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis - VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1 mutants segregate independently of the vtc2-1 mutation. These observations suggest that it is the presence of an independent cryptic mutation that affects growth of vtc2-1 mutants, and not the 70-80% decrease in ascorbate levels that has been assumed in past studies.

Citing Articles

Chloroplastic ascorbate modifies plant metabolism and may act as a metabolite signal regardless of oxidative stress.

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Localization data of the T-DNA insertion site in Arabidopsis line SALK_146824C.

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Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.

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