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» Articles » PMID: 27467068

Safety Assessment of Two New Lactobacillus Strains As Probiotic for Human Using a Rat Model

Overview
Journal PLoS One
Specialties General Medicine
Science
Date 2016 Jul 29
PMID 27467068
Citations 23
Authors
Parisa Shokryazdan
Mohammad Faseleh Jahromi
Juan Boo Liang
Ramasamy Kalavathy
Chin Chin Sieo
Yin Wan Ho
Affiliations
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Abstract

Two previously isolated Lactobacillus strains (L. fermentum HM3 from human milk and L. buchneri FD2 from fermented dates), intended as probiotic for human, were assessed for their safety using acute and subacute oral toxicity tests in rats. In addition, their effects on cecal microflora and harmful bacterial enzymes (β-glucuronidase and β-glucosidase) of the tested animals were also determined. The results showed that L. buchneri FD2, L. fermentum HM3, or a mixture of them were safe up to a level of 1010 CFU/kg BW/day in a 14-day or 28-day treatment period. Both strains were well tolerated and there were no observed adverse effects on growth, feed consumption, cellular blood components and vital organs of the treated animals. The Lactobacillus strains were also able to reduce harmful intestinal bacterial enzymes, and decrease pathogenic bacterial populations while increasing beneficial bacterial populations. These results suggest that the two Lactobacillus strains are safe and could be potential probiotic for human.

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