ER Network Homeostasis is Critical for Plant Endosome Streaming and Endocytosis

Overview

Journal Cell Discov

Date 2016 Jul 28

PMID 27462431

Citations 20

Authors

Giovanni Stefano

Luciana Renna

YaShiuan Lai

Erin Slabaugh

Nicole Mannino

Rafael A Buono

Marisa S Otegui

Federica Brandizzi

Affiliations

Soon will be listed here.

Abstract

Eukaryotic cells internalize cargo at the plasma membrane via endocytosis, a vital process that is accomplished through a complex network of endosomal organelles. In mammalian cells, the ER is in close association with endosomes and regulates their fission. Nonetheless, the physiological role of such interaction on endocytosis is yet unexplored. Here, we probed the existence of ER-endosome association in plant cells and assayed its physiological role in endocytosis. Through live-cell imaging and electron microscopy studies, we established that endosomes are extensively associated with the plant ER, supporting conservation of interaction between heterotypic organelles in evolutionarily distant kingdoms. Furthermore, by analyzing ER-endosome dynamics in genetic backgrounds with defects in ER structure and movement, we also established that the ER network integrity is necessary for homeostasis of the distribution and streaming of various endosome populations as well as for efficient endocytosis. These results support a novel model that endocytosis homeostasis depends on a spatiotemporal control of the endosome dynamics dictated by the ER membrane network.

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