MiR-34C Disrupts the Stemness of Purified CD133 Prostatic Cancer Stem Cells

Overview

Journal Urology

Specialty Urology

Date 2016 Jul 28

PMID 27461446

Citations 7

Authors

Yuan Chen

Qun Rao

Huiping Zhang

Hua Xu

Cuntai Zhang

Qianyuan Zhuang

Zhangqun Ye

Affiliations

Soon will be listed here.

Abstract

Objective: To find the potential micro-RNA (miRNA) that could determine the fate of prostate cancer stem cells.

Materials And Methods: We compared miRNA expression between our purified CD133 prostatic cancer stem cells (PCSCs) and CD133 cells. Sphere formation assay and matrigel-based cell invasion assay were applied to determine the stemness of CD133 PCSCs after our manipulation of miRNA using miRNA mimic or miRNA inhibitor.

Results: In this study, we identified that miR-34C was under-expressed in the purified CD133 PCSCs and enforced introduction of miR-34C attenuated the stemness of CD133 PCSCs. Clinically, we also observed a negative correlation between miR-34C and CD133.

Conclusion: Our data strongly suggest that miR-34C may play essential role in conferring castration resistance by equilibrating PSCS population.

Citing Articles

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Giridharan M, Rupani V, Banerjee S ACS Pharmacol Transl Sci. 2022; 5(4):193-206.

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MicroRNA-34c-3p target inhibiting NOTCH1 suppresses chemosensitivity and metastasis of non-small cell lung cancer.

Yang L, Lei C, Zhao Y, Sun H, Yu Q, Yang E J Int Med Res. 2020; 48(3):300060520904847.

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