Spontaneous In Vivo Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling

Overview

Journal Stem Cells Transl Med

Publisher Oxford University Press

Specialties Cell Biology
Pharmacology

Date 2016 Jul 28

PMID 27460852

Citations 22

Authors

Anna Marsano

Carolina M Medeiros Da Cunha

Shahram Ghanaati

Sinan Gueven

Matteo Centola

Roman Tsaryk

Mike Barbeck

Chiara Stuedle

Andrea Barbero

Uta Helmrich

Stefan Schaeren

James C Kirkpatrick

Andrea Banfi

Ivan Martin

Affiliations

Soon will be listed here.

Abstract

Significance: Chondrogenic differentiation of mesenchymal stromal/stem cells (MSCs) is typically targeted by morphogen delivery, which is often associated with limited efficiency, stability, and robustness. This article proposes a strategy to engineer MSCs with the capacity to establish specific microenvironmental conditions, supporting their own targeted differentiation program. Sole blockade of angiogenesis mediated by transduction for sFlk-1, without delivery of additional morphogens, is sufficient for inducing MSC chondrogenic differentiation. The findings represent a relevant step forward in the field because the method allowed reducing interdonor variability in MSC differentiation efficiency and, importantly, onset of a stable, nonhypertrophic chondrocyte phenotype.

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