Indication of Nerve Growth Factor Binding Components from Herbal Extracts by HerboChip: a Platform for Drug Screening on a Chip

Overview

Journal Chin Med

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2016 Jul 26

PMID 27453720

Citations 4

Authors

Pinky Sum Chi Lee

Laura Minglu Zhang

Artemis Lu Yan

Kelly Yin Ching Lam

Tina Tingxia Dong

Huangquan Lin

Gallant Kar Lun Chan

Karl Wah Keung Tsim

Affiliations

Soon will be listed here.

Abstract

Background: HerboChip is an array of different fractions deriving from herbal extracts. This study aimed to identify effective components from Chinese medicine (CM) that interact with nerve growth factor (NGF) as a target using HerboChip.

Methods: Fifty types of CM that are traditionally used as remedies for emotion imbalance were selected and extracted with 50 % ethanol. Biotinylated-NGF was hybridized with over 300 chips coated with different HPLC-separated fractions from CM extracts and straptavidin-Cy5 was used to identify the NGF-bound fractions.

Results: Over 300 chips were screened within a week, and 17 positive hits were identified. The interaction of the identified herbal extracts with NGF was confirmed in cultured PC12 cells. Co-application of NGF and herbal extract interfered with NGF-induced expression of neurofilaments, including NF68 and NF200 in cell cultures. Western blot analysis comparing the intensity of phosphorylated cAMP response element-binding protein (CREB) over total CREB showed NGF-induced CREB phosphorylation was modulated by the identified herbal extracts. Five CM herbs showed activating activities on the NGF response and nine CM herbs showed inhibiting activities.

Conclusion: The current result supported the applicability of HerboChip for screening NGF binding components from herbal extracts.

Citing Articles

Screening of herbal extracts binding with vascular endothelial growth factor by applying HerboChip platform.

Liu Y, Liang J, Guo G, Qiu Y, Yu L, Tsim K Chin Med. 2024; 19(1):122.

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A Herbal Mixture of Sesami Semen Nigrum and Longan Arillus Induces Neurite Outgrowth in Cultured Neurons and Shows Anti-Depression in Chronic Mild Stress-Induced Rats.

Gao A, Xia T, Mak M, Zhu K, Dong T, Tsim K Evid Based Complement Alternat Med. 2022; 2022:8809546.

PMID: 35754681 PMC: 9225919. DOI: 10.1155/2022/8809546.

Kaempferol, a Major Flavonoid in Ginkgo Folium, Potentiates Angiogenic Functions in Cultured Endothelial Cells by Binding to Vascular Endothelial Growth Factor.

Hu W, Wang H, Xia Y, Dai D, Xiong Q, Dong T Front Pharmacol. 2020; 11:526.

PMID: 32410995 PMC: 7198864. DOI: 10.3389/fphar.2020.00526.

Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF.

Chan G, Hu W, Zheng Z, Huang M, Lin Y, Wang C Evid Based Complement Alternat Med. 2018; 2018:1502457.

PMID: 29681968 PMC: 5850895. DOI: 10.1155/2018/1502457.

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