Isolation and Characterization of Helicobacter Pylori Recovered from Gastric Biopsies Under Anaerobic Conditions

Overview

Journal Diagn Microbiol Infect Dis

Specialties Infectious Diseases
Microbiology
Pathology

Date 2016 Jul 26

PMID 27452640

Citations 4

Authors

Guillerm Ignacio Perez-Perez

Thinh Nguyen Van

Duong Thu Huong

Gao Zhan

Do Nguyet Anh

Nguyen Thi Nguyet

Loan Ta Thi

Nguyen Van Thinh

Nguyen Thi Hong-Hanh

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients.

Methods: We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR.

Results: H. pylori were recovered under anaerobic and micro-aerobic conditions from gastric biopsies in 16 patients. Anaerobic conditions yielded significantly higher H. pylori recovery rates than micro-aerobic conditions (81.3% vs. 31.3%, P= 0.01). H. pylori isolates were characterized by PCR for specific virulence markers and the genotypes were similar to those previously described in this region of the world.

Conclusions: H. pylori can be isolated under anaerobic conditions. These findings may provide new insight into the physiology of this human pathogen and help to identify the route of H. pylori transmission.

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References

Gao Z, Tseng C, Pei Z, Blaser M . Molecular analysis of human forearm superficial skin bacterial biota. Proc Natl Acad Sci U S A. 2007; 104(8):2927-32. PMC: 1815283. DOI: 10.1073/pnas.0607077104. View

Yamaoka Y, Kato M, Asaka M . Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med. 2008; 47(12):1077-83. PMC: 3732488. DOI: 10.2169/internalmedicine.47.0975. View

Agarwal S, Jithendra K . Presence of Helicobacter pylori in subgingival plaque of periodontitis patients with and without dyspepsia, detected by polymerase chain reaction and culture. J Indian Soc Periodontol. 2012; 16(3):398-403. PMC: 3498711. DOI: 10.4103/0972-124X.100919. View

Kim S . Small intestine transit time in the normal small bowel study. Am J Roentgenol Radium Ther Nucl Med. 1968; 104(3):522-4. DOI: 10.2214/ajr.104.3.522. View

Goodwin C, Armstrong J . Microbiological aspects of Helicobacter pylori (Campylobacter pylori). Eur J Clin Microbiol Infect Dis. 1990; 9(1):1-13. DOI: 10.1007/BF01969526. View

Nomura A, STEMMERMANN G, Chyou P, Kato I, Perez-Perez G, Blaser M . Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med. 1991; 325(16):1132-6. DOI: 10.1056/NEJM199110173251604. View

Kidd M, Modlin I . A century of Helicobacter pylori: paradigms lost-paradigms regained. Digestion. 1998; 59(1):1-15. DOI: 10.1159/000007461. View

Momtaz H, Souod N, Dabiri H, Sarshar M . Study of Helicobacter pylori genotype status in saliva, dental plaques, stool and gastric biopsy samples. World J Gastroenterol. 2012; 18(17):2105-11. PMC: 3342610. DOI: 10.3748/wjg.v18.i17.2105. View

Bury-Mone S, Kaakoush N, Asencio C, Megraud F, Thibonnier M, De Reuse H . Is Helicobacter pylori a true microaerophile?. Helicobacter. 2006; 11(4):296-303. DOI: 10.1111/j.1523-5378.2006.00413.x. View

10.

Megraud F . Transmission of Helicobacter pylori: faecal-oral versus oral-oral route. Aliment Pharmacol Ther. 1995; 9 Suppl 2:85-91. View

11.

Hatakeyama M, Higashi H . Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis. Cancer Sci. 2005; 96(12):835-43. PMC: 11159386. DOI: 10.1111/j.1349-7006.2005.00130.x. View

12.

Van Duynhoven Y, de Jonge R . Transmission of Helicobacter pylori: a role for food?. Bull World Health Organ. 2001; 79(5):455-60. PMC: 2566425. View

13.

Ghoshal U, Sengar V, Srivastava D . Colonic Transit Study Technique and Interpretation: Can These Be Uniform Globally in Different Populations With Non-uniform Colon Transit Time?. J Neurogastroenterol Motil. 2012; 18(2):227-8. PMC: 3325313. DOI: 10.5056/jnm.2012.18.2.227. View

14.

Cave D . Transmission and epidemiology of Helicobacter pylori. Am J Med. 1996; 100(5A):12S-17S; discussion 17S-18S. DOI: 10.1016/s0002-9343(96)80224-5. View

15.

Megraud F . The most important diagnostic modalities for Helicobacter pylori, now and in the future. Eur J Gastroenterol Hepatol. 2012; 9 Suppl 1:S13-5. View

16.

Nguyen T, Uchida T, Tsukamoto Y, Trinh D, Ta L, Mai B . Helicobacter pylori infection and gastroduodenal diseases in Vietnam: a cross-sectional, hospital-based study. BMC Gastroenterol. 2010; 10:114. PMC: 2959090. DOI: 10.1186/1471-230X-10-114. View

17.

McNulty S, Mole B, Dailidiene D, Segal I, Ally R, Mistry R . Novel 180- and 480-base-pair insertions in African and African-American strains of Helicobacter pylori. J Clin Microbiol. 2004; 42(12):5658-63. PMC: 535299. DOI: 10.1128/JCM.42.12.5658-5663.2004. View

18.

West A, Millar M, Tompkins D . Effect of physical environment on survival of Helicobacter pylori. J Clin Pathol. 1992; 45(3):228-31. PMC: 495480. DOI: 10.1136/jcp.45.3.228. View

19.

Chung C, Olivares A, Torres E, Yilmaz O, Cohen H, Perez-Perez G . Diversity of VacA intermediate region among Helicobacter pylori strains from several regions of the world. J Clin Microbiol. 2010; 48(3):690-6. PMC: 2832466. DOI: 10.1128/JCM.01815-09. View

20.

Yamaguchi H, Osaki T, Takahashi M, Taguchi H, Kamiya S . Colony formation by Helicobacter pylori after long-term incubation under anaerobic conditions. FEMS Microbiol Lett. 1999; 175(1):107-11. DOI: 10.1111/j.1574-6968.1999.tb13608.x. View