ABCG2 is Required for Self-renewal and Chemoresistance of CD133-positive Human Colorectal Cancer Cells

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2016 Jul 25

PMID 27449042

Citations 19

Authors

Lijun Ma

Ting Liu

Yiran Jin

Jun Wei

Yinxue Yang

Hongquan Zhang

Affiliations

Soon will be listed here.

Abstract

There is increasing evidence supporting the cancer stem cell (CSC) hypothesis, which suggests that a population of tumor cells with stem cell characteristics is responsible for tumor growth, resistance, and recurrence as well as drug resistance. In colorectal cancer, the CD133 antigen defines distinct cell subpopulations that are rich in tumor-initiating cells; however, the drug resistance properties of these CD133-positive cells have not been well defined. The breast cancer resistance protein (BCRP)/ATP-binding cassette subfamily G member 2 (ABCG2) is present on the plasma membrane of many types of human cancer cells and contributes to multidrug resistance during chemotherapy. The results of the present study showed that ABCG2 is expressed in CD133-positive CSCs from human colorectal tumors. Furthermore, the downregulation of ABCG2 expression inhibited the self-renewal capacity of these cells, and significantly enhanced the efficacy of chemotherapy-induced apoptosis in LS174T colon adenocarcinoma cells and CD133-positive colorectal carcinoma cells. Together, these data show that ABCG2 expression correlates with the presence of CD133-positive cancer cells, and thus is a possible therapeutic target for colorectal cancer.

Citing Articles

Self-Renewal Inhibition in Breast Cancer Stem Cells: Moonlight Role of PEDF in Breast Cancer.

Gil-Gas C, Sanchez-Diez M, Honrubia-Gomez P, Sanchez-Sanchez J, Alvarez-Simon C, Sabater S Cancers (Basel). 2023; 15(22).

PMID: 38001682 PMC: 10670784. DOI: 10.3390/cancers15225422.

Gene and ABCG2 Protein Expression in Colorectal Cancer-In Silico and Wet Analysis.

Salagacka-Kubiak A, Zawada D, Saed L, Kordek R, Jelen A, Balcerczak E Int J Mol Sci. 2023; 24(13).

PMID: 37445716 PMC: 10341438. DOI: 10.3390/ijms241310539.

Intense endoplasmic reticulum stress (ERS) / IRE1α enhanced Oxaliplatin efficacy by decreased ABCC10 in colorectal cancer cells.

Liu X, Wu B, Chen H, Sun H, Guo X, Sun T BMC Cancer. 2022; 22(1):1369.

PMID: 36585626 PMC: 9805014. DOI: 10.1186/s12885-022-10415-8.

Expression of Chemoresistance-Associated ABC Proteins in Hepatobiliary, Pancreatic and Gastrointestinal Cancers.

Marin J, Monte M, Macias R, Romero M, Herraez E, Asensio M Cancers (Basel). 2022; 14(14).

PMID: 35884584 PMC: 9320734. DOI: 10.3390/cancers14143524.

Implication of Different Tumor Biomarkers in Drug Resistance and Invasiveness in Primary and Metastatic Colorectal Cancer Cell Lines.

Sanchez-Diez M, Alegria-Aravena N, Lopez-Montes M, Quiroz-Troncoso J, Gonzalez-Martos R, Menendez-Rey A Biomedicines. 2022; 10(5).

PMID: 35625820 PMC: 9139065. DOI: 10.3390/biomedicines10051083.

References

Horst D, Scheel S, Liebmann S, Neumann J, Maatz S, Kirchner T . The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer. J Pathol. 2009; 219(4):427-34. DOI: 10.1002/path.2597. View

Garson K, Vanderhyden B . Epithelial ovarian cancer stem cells: underlying complexity of a simple paradigm. Reproduction. 2014; 149(2):R59-70. DOI: 10.1530/REP-14-0234. View

Todaro M, Perez Alea M, Di Stefano A, Cammareri P, Vermeulen L, Iovino F . Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4. Cell Stem Cell. 2008; 1(4):389-402. DOI: 10.1016/j.stem.2007.08.001. View

Chen K, Huang Y, Chen J . Understanding and targeting cancer stem cells: therapeutic implications and challenges. Acta Pharmacol Sin. 2013; 34(6):732-40. PMC: 3674516. DOI: 10.1038/aps.2013.27. View

OBrien C, Pollett A, Gallinger S, Dick J . A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature. 2006; 445(7123):106-10. DOI: 10.1038/nature05372. View

Liang S, Yang C, Tseng J, Wang H, Tung C, Liu H . ABCG2 localizes to the nucleus and modulates CDH1 expression in lung cancer cells. Neoplasia. 2015; 17(3):265-78. PMC: 4372652. DOI: 10.1016/j.neo.2015.01.004. View

Saigusa S, Tanaka K, Toiyama Y, Yokoe T, Okugawa Y, Ioue Y . Correlation of CD133, OCT4, and SOX2 in rectal cancer and their association with distant recurrence after chemoradiotherapy. Ann Surg Oncol. 2009; 16(12):3488-98. DOI: 10.1245/s10434-009-0617-z. View

Bhattacharya S, Das A, Mallya K, Ahmad I . Maintenance of retinal stem cells by Abcg2 is regulated by notch signaling. J Cell Sci. 2007; 120(Pt 15):2652-62. DOI: 10.1242/jcs.008417. View

Yan H, Fang L, Xu J, Qiu Y, Lin X, Huang H . The identification of the biological characteristics of human ovarian cancer stem cells. Eur Rev Med Pharmacol Sci. 2014; 18(22):3497-503. View

10.

Hongo K, Tanaka J, Tsuno N, Kawai K, Nishikawa T, Shuno Y . CD133(-) cells, derived from a single human colon cancer cell line, are more resistant to 5-fluorouracil (FU) than CD133(+) cells, dependent on the β1-integrin signaling. J Surg Res. 2011; 175(2):278-88. DOI: 10.1016/j.jss.2011.03.076. View

11.

Chen H, Zhao J, Qian X, Cheng Z, Liu Y, Wang Z . RASAL1 attenuates gastric carcinogenesis in nude mice by blocking RAS/ERK signaling. Asian Pac J Cancer Prev. 2015; 16(3):1077-82. DOI: 10.7314/apjcp.2015.16.3.1077. View

12.

Lee M, Ji S, Mia-Jan K, Cho M . Chemoresistance of CD133(+) colon cancer may be related with increased survivin expression. Biochem Biophys Res Commun. 2015; 463(3):229-34. DOI: 10.1016/j.bbrc.2015.05.031. View

13.

Doyle L, Yang W, Abruzzo L, Krogmann T, Gao Y, Rishi A . A multidrug resistance transporter from human MCF-7 breast cancer cells. Proc Natl Acad Sci U S A. 1998; 95(26):15665-70. PMC: 28101. DOI: 10.1073/pnas.95.26.15665. View

14.

Gao X, Mi Y, Ma Y, Jin W . LEF1 regulates glioblastoma cell proliferation, migration, invasion, and cancer stem-like cell self-renewal. Tumour Biol. 2014; 35(11):11505-11. DOI: 10.1007/s13277-014-2466-z. View

15.

Honjo Y, Hrycyna C, Yan Q, Robey R, van de Laar A, Litman T . Acquired mutations in the MXR/BCRP/ABCP gene alter substrate specificity in MXR/BCRP/ABCP-overexpressing cells. Cancer Res. 2001; 61(18):6635-9. View

16.

Polgar O, Robey R, Bates S . ABCG2: structure, function and role in drug response. Expert Opin Drug Metab Toxicol. 2008; 4(1):1-15. DOI: 10.1517/17425255.4.1.1. View

17.

Yang J, Liu Y, Zhong W, Yu D, Wen L, Jin C . Chemoresistance of CD133+ cancer stem cells in laryngeal carcinoma. Chin Med J (Engl). 2011; 124(7):1055-60. View

18.

Mannelli G, Magnelli L, Deganello A, Busoni M, Meccariello G, Parrinello G . Detection of putative stem cell markers, CD44/CD133, in primary and lymph node metastases in head and neck squamous cell carcinomas. A preliminary immunohistochemical and in vitro study. Clin Otolaryngol. 2015; 40(4):312-20. DOI: 10.1111/coa.12368. View

19.

Artells R, Moreno I, Diaz T, Martinez F, Gel B, Navarro A . Tumour CD133 mRNA expression and clinical outcome in surgically resected colorectal cancer patients. Eur J Cancer. 2009; 46(3):642-9. DOI: 10.1016/j.ejca.2009.11.003. View

20.

Vermeulen L, Todaro M, de Sousa Mello F, Sprick M, Kemper K, Alea M . Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity. Proc Natl Acad Sci U S A. 2008; 105(36):13427-32. PMC: 2533206. DOI: 10.1073/pnas.0805706105. View