Metabolomics Analysis for Hydroxy-L-proline-induced Calcium Oxalate Nephrolithiasis in Rats Based on Ultra-high Performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jul 23

PMID 27443631

Citations 10

Authors

Songyan Gao

Rui Yang

Zhongjiang Peng

Hongtao Lu

Na Li

Jiarong Ding

Xingang Cui

Wei Chen

Xin Dong

Affiliations

Soon will be listed here.

Abstract

About 80% of kidney stones are composed of calcium oxalate (CaOx) with variable amounts of calcium phosphate, and hyperoxaluria is considered as an important factor of CaOx nephrolithiasis. However, the underlying metabolic mechanisms of CaOx nephrolithiasis remain undefined. In this study, we successfully developed a rat model with hydroxy-L-proline (HLP) -induced CaOx nephrolithiasis. Rats were continuously orally administrated with HLP for 28 days. Urine and blood samples were collected from the rats treated with or without HLP at four different time points. UPLC-Q-TOF/MS was applied to profile the abundances of metabolites. To obtain more comprehensive analysis of metabolic profiling spectrum, combination of RP-LC and HILIC were applied. We identify 42 significant differential metabolites in the urine, and 13 significant differential metabolites in the blood. Pathway analysis revealed that the pathways involved in amino acid metabolism, taurine metabolism, bile acid synthesis, energy metabolism, TCA cycle, purine metabolism, vitamin metabolism, nicotinic acid and nicotinamide metabolism have been modulated by HLP treatment. This study suggested that a number of metabolic pathways are dysfunctional in the HLP induced crystal kidney injury, and further studies on those pathways are warranted to better understand the metabolic mechanism of CaOx nephrolithiasis.

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