Low Acrosin Activity in a Subgroup of Men with Idiopathic Infertility Dose Not Correlate with Sperm Density, Percent Motility, Curvilinear Velocity, or Linearity

The authors compared sperm acrosin activity with sperm density and cell motion characteristics in 21 normal fertile men and 25 patients with unexplained infertility. Under standardized and optimized conditions of abstinence and semen sample processing, we measured sperm acrosin activity in washed sperm from direct aliquots of semen and in aliquots of semen filtered through glasswool to remove dead cells and debris. Using washed sperm from semen, sperm acrosin levels in infertile men (median, 44 microIU/10(6) sperm) were significantly lower than values measured in fertile men (median, 67 microIU/10(6) sperm, P less than 0.01). After glasswool filtration, sperm acrosin activity was higher for both fertile and infertile men. Using washed sperm, 7 of 25 patients had acrosin activity consistently below values measured for fertile men; after glasswool filtration, values for 8 of 14 patients were below the normal range. For either fertile or infertile men, sperm acrosin activity showed no correlation with sperm density, percent motility, or either motion characteristic of curvilinear velocity (Vc40 microns/sec) or linearity (L3); and further, the low sperm acrosin activity of some infertile patients did not correlate with the motion co-characteristics measured at Vc40/L3, and the majority of patients with slower and/or less directional sperm had normal acrosin activity. From our data, we therefore conclude that sperm acrosin activity is independent of sperm motion characteristics.