Accumulation of Arachidonic Acid-containing Phosphatidylinositol at the Outer Edge of Colorectal Cancer

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jul 21

PMID 27435310

Citations 25

Authors

Takanori Hiraide

Koji Ikegami

Takanori Sakaguchi

Yoshifumi Morita

Takahiro Hayasaka

Noritaka Masaki

Michihiko Waki

Eiji Sugiyama

Satoru Shinriki

Makoto Takeda

Yasushi Shibasaki

Shinichiro Miyazaki

Hirotoshi Kikuchi

Hiroaki Okuyama

Masahiro Inoue

Mitsutoshi Setou

Hiroyuki Konno

Affiliations

Soon will be listed here.

Abstract

Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edge-specific lipid compositions emerge autonomously in cancer cells. Cancer tissue-originated spheroids (CTOSs) that are composed of pure primary cancer cells have been developed. In this study, we aimed to seek out the cancer cell-autonomous acquisition of cancer outer edge-characterizing lipids in colorectal cancer by analysing phospholipids in CTOSs derived from colorectal cancer patients with matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). A signal at m/z 885.5 in negative ion mode was detected specifically at the surface regions. The signal was identified as an arachidonic acid (AA)-containing phosphatidylinositol (PI), PI(18:0/20:4), by tandem mass spectrometry analysis. Quantitative analysis revealed that the amount of PI(18:0/20:4) in the surface region of CTOSs was two-fold higher than that in the medial region. Finally, PI(18:0/20:4) was enriched at the cancer cells/stromal interface in colorectal cancer patients. These data imply a possible importance of AA-containing PI for colorectal cancer progression, and suggest cells expressing AA-containing PI as potential targets for anti-cancer therapy.

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