Diet-induced Elevation of Circulating HSP70 May Trigger Cell Adhesion and Promote the Development of Atherosclerosis in Rats

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2016 Jul 21

PMID 27435079

Citations 13

Authors

Fang Xie

Rui Zhan

Li-Cheng Yan

Jing-Bo Gong

Yun Zhao

Jing Ma

Ling-Jia Qian

Affiliations

Soon will be listed here.

Abstract

Although accumulating evidence indicates that heat shock protein 70 (HSP70) could be secreted into plasma and its levels have been found to have an ambiguous association with atherosclerosis, our knowledge for the exact role of circulating HSP70 in the development of atherosclerosis is still limited. In the present study, we report an adhesion-promoting effect of exogenous HSP70 and evaluate the potential involvement of elevated circulating HSP70 in the development of atherosclerosis. Time-dependent elevation of plasma HSP70 was found in diet-induced atherosclerotic rats, whose effect was investigated through further in vitro experiments. In rat aortic endothelial cell (RAEC) cultures, exogenous HSP70 incubation neither produced cell injuries by itself nor had protective effects on cell injuries caused by Ox-LDL or homocysteine. However, exogenous HSP70 administration could lead to a higher adhesion rate between rat peripheral blood monocytes (PBMCs) and RAECs. This adhesion-promoting effect appeared only when PBMCs, rather than RAECs, were pretreated with HSP70 incubation. PBMCs in an HSP70 environment released more IL-6 to supernatant, which subsequently up-regulated the expression of ICAM-1 in RAECs. These results indicate that the diet-induced elevation of circulating HSP70 could trigger cell adhesion with the help of IL-6 as a mediator, which provides a novel possible mechanism for understanding the role of circulating HSP70 in the pathogenesis of atherosclerosis.

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References

Asea A, Rehli M, Kabingu E, Boch J, Bare O, Auron P . Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4. J Biol Chem. 2002; 277(17):15028-34. DOI: 10.1074/jbc.M200497200. View

Laulagnier K, Motta C, Hamdi S, Roy S, Fauvelle F, Pageaux J . Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization. Biochem J. 2004; 380(Pt 1):161-71. PMC: 1224152. DOI: 10.1042/BJ20031594. View

Wendling U, Paul L, van der Zee R, Prakken B, Singh M, van Eden W . A conserved mycobacterial heat shock protein (hsp) 70 sequence prevents adjuvant arthritis upon nasal administration and induces IL-10-producing T cells that cross-react with the mammalian self-hsp70 homologue. J Immunol. 2000; 164(5):2711-7. DOI: 10.4049/jimmunol.164.5.2711. View

Krepuska M, Szeberin Z, Sotonyi P, Sarkadi H, Fehervari M, Apor A . Serum level of soluble Hsp70 is associated with vascular calcification. Cell Stress Chaperones. 2010; 16(3):257-65. PMC: 3077222. DOI: 10.1007/s12192-010-0237-3. View

Xu Q . Biomechanical-stress-induced signaling and gene expression in the development of arteriosclerosis. Trends Cardiovasc Med. 2001; 10(1):35-41. DOI: 10.1016/s1050-1738(00)00042-6. View

Hunter-Lavin C, Davies E, Bacelar M, Marshall M, Andrew S, Williams J . Hsp70 release from peripheral blood mononuclear cells. Biochem Biophys Res Commun. 2004; 324(2):511-7. DOI: 10.1016/j.bbrc.2004.09.075. View

Calderwood S, Mambula S, Gray Jr P, Theriault J . Extracellular heat shock proteins in cell signaling. FEBS Lett. 2007; 581(19):3689-94. DOI: 10.1016/j.febslet.2007.04.044. View

Mambula S, Calderwood S . Heat shock protein 70 is secreted from tumor cells by a nonclassical pathway involving lysosomal endosomes. J Immunol. 2006; 177(11):7849-57. DOI: 10.4049/jimmunol.177.11.7849. View

Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M . Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol. 2001; 166(3):2018-24. DOI: 10.4049/jimmunol.166.3.2018. View

10.

Yokota S, Minota S, Fujii N . Anti-HSP auto-antibodies enhance HSP-induced pro-inflammatory cytokine production in human monocytic cells via Toll-like receptors. Int Immunol. 2006; 18(4):573-80. DOI: 10.1093/intimm/dxh399. View

11.

Galovic R, Flegar-Mestric Z, Vidjak V, Matokanovic M, Barisic K . Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis. Clin Biochem. 2015; 49(1-2):66-9. DOI: 10.1016/j.clinbiochem.2015.10.006. View

12.

Cai G, Miao C, Xie H, Lu L, Su D . Arterial baroreflex dysfunction promotes atherosclerosis in rats. Atherosclerosis. 2005; 183(1):41-7. DOI: 10.1016/j.atherosclerosis.2005.03.037. View

13.

Deanfield J, Halcox J, Rabelink T . Endothelial function and dysfunction: testing and clinical relevance. Circulation. 2007; 115(10):1285-95. DOI: 10.1161/CIRCULATIONAHA.106.652859. View

14.

Lipsker D, Ziylan U, Spehner D, Proamer F, Bausinger H, Jeannin P . Heat shock proteins 70 and 60 share common receptors which are expressed on human monocyte-derived but not epidermal dendritic cells. Eur J Immunol. 2002; 32(2):322-32. DOI: 10.1002/1521-4141(200202)32:2<322::AID-IMMU322>3.0.CO;2-0. View

15.

Wick G, Jakic B, Buszko M, Wick M, Grundtman C . The role of heat shock proteins in atherosclerosis. Nat Rev Cardiol. 2014; 11(9):516-29. DOI: 10.1038/nrcardio.2014.91. View

16.

Johnson A, Berberian P, Tytell M, Bond M . Differential distribution of 70-kD heat shock protein in atherosclerosis. Its potential role in arterial SMC survival. Arterioscler Thromb Vasc Biol. 1995; 15(1):27-36. DOI: 10.1161/01.atv.15.1.27. View

17.

Wright B, Corton J, El-Nahas A, Wood R, Pockley A . Elevated levels of circulating heat shock protein 70 (Hsp70) in peripheral and renal vascular disease. Heart Vessels. 2000; 15(1):18-22. DOI: 10.1007/s003800070043. View

18.

Martin-Ventura J, Leclercq A, Blanco-Colio L, Egido J, Rossignol P, Meilhac O . Low plasma levels of HSP70 in patients with carotid atherosclerosis are associated with increased levels of proteolytic markers of neutrophil activation. Atherosclerosis. 2006; 194(2):334-41. DOI: 10.1016/j.atherosclerosis.2006.10.030. View

19.

Xu Q, Metzler B, Jahangiri M, Mandal K . Molecular chaperones and heat shock proteins in atherosclerosis. Am J Physiol Heart Circ Physiol. 2011; 302(3):H506-14. PMC: 3353778. DOI: 10.1152/ajpheart.00646.2011. View

20.

Schuett H, Luchtefeld M, Grothusen C, Grote K, Schieffer B . How much is too much? Interleukin-6 and its signalling in atherosclerosis. Thromb Haemost. 2009; 102(2):215-22. DOI: 10.1160/TH09-05-0297. View