Methylation Status of H19/IGF2 Differentially Methylated Region in in Vitro Human Blastocysts Donated by Healthy Couples

Overview

Journal Iran Biomed J

Specialties Biology
General Medicine

Date 2016 Jul 20

PMID 27432596

Authors

Marzieh Derakhshan-Horeh

Farid Abolhassani

Farnoosh Jafarpour

Ashraf Moini

Khadijeh Karbalaie

Sayyed Morteza Hosseini

Somayyeh Ostadhosseini

Mohammad Hossein Nasr-Esfahani

Affiliations

Soon will be listed here.

Abstract

Background: Imprinted genes are a unique subset of few genes that have been differentially methylated region (DMR) in a parental origin-dependent manner during gametogenesis, and these genes are highly protected during pre-implantation epigenetic reprogramming. Several studies have shown that the particular vulnerability of imprinting genes during suboptimal pre- and peri-conception micro-environments often is occurred by assisted reproduction techniques (ART). This study investigated the methylation status of H19/IGF2 DMR at high-quality expanding/expanded human blastocysts donated by healthy individuals to evaluate the risks linked to ART.

Methods: Methylation levels of H19/IGF2 DMR were analyzed by bisulfite conversion and sequencing at 18 CpG sites (CpGs) located in this region.

Results: The overall percentage of methylated CpGs and the proportion of hyper-methylated clones of H19/IGF2 DMR in analyzed blastocysts were 37.85±4.87% and 43.75±5.1%, respectively. For validation of our technique, the corresponding methylation levels of peripheral human lymphocytes were defined (49.52±1.86% and 50%, respectively).

Conclusions: Considering the absence of in vivo- produced human embryos, it is not possible to conclude that the methylation found in H19/IGF2 DMR is actually normal or abnormal. Regarding the possible risks associated with ART, the procedures should be optimized in order to at least reduce some of the epigenetic risks.

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