Fluorogenic Probes for Multicolor Imaging in Living Cells

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2016 Jul 16

PMID 27420907

Citations 69

Authors

Grazvydas Lukinavicius

Luc Reymond

Keitaro Umezawa

Olivier Sallin

Elisa DEste

Fabian Gottfert

Haisen Ta

Stefan W Hell

Yasuteru Urano

Kai Johnsson

Affiliations

Soon will be listed here.

Abstract

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.

Citing Articles

Uptake of small extracellular vesicles by recipient cells is facilitated by paracrine adhesion signaling.

Hirosawa K, Sato Y, Kasai R, Yamaguchi E, Komura N, Ando H Nat Commun. 2025; 16(1):2419.

PMID: 40075063 PMC: 11903687. DOI: 10.1038/s41467-025-57617-9.

Wash-free fluorescent tools based on organic molecules: Design principles and biomedical applications.

Tan J, Wang C, Hu Z, Zhang X Exploration (Beijing). 2025; 5(1):20230094.

PMID: 40040824 PMC: 11875451. DOI: 10.1002/EXP.20230094.

Lipid-Directed Covalent Labeling of Plasma Membranes for Long-Term Imaging, Barcoding and Manipulation of Cells.

Aknine N, Pelletier R, Klymchenko A JACS Au. 2025; 5(2):922-936.

PMID: 40017781 PMC: 11863151. DOI: 10.1021/jacsau.4c01134.

multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors.

Zheng Y, Cai R, Wang K, Zhang J, Zhuo Y, Dong H bioRxiv. 2025; .

PMID: 39763912 PMC: 11703222. DOI: 10.1101/2024.12.22.629999.

Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.

Ortega R, Fernandez-Monreal M, Pied N, Roudeau S, Cloetens P, Carmona A Chem Biomed Imaging. 2024; 2(11):744-754.

PMID: 39610464 PMC: 11600181. DOI: 10.1021/cbmi.4c00038.