[17β‑estradiol Suppresses Hyperoxia‑induced Apoptosis of Oligodendrocyte Precursor Cells Through Paired‑immunoglobulin‑like Receptor B]

Overview

Journal Zhongguo Dang Dai Er Ke Za Zhi

Specialty Pediatrics

Date 2016 Jul 15

PMID 27412551

Authors

Hua Wang

De-Zhi Mu

Affiliations

Soon will be listed here.

Abstract

Objective: To study the effect of hyperoxia and paired immunoglobin-like receptor B (PirB) on rat oligodendrocyte precursor cells (OPCs) in vivo and the neuroprotective effects of 17β-estradiol (E2) on these cells.

Methods: Rat OPCs were treated with different concentrations of E2 and the cells were harvested for RT‑qPCR analysis at different time points. PriB was silenced with small interfering siRNA. The effects of E2 treatment and silencing of PriB on OPCs viability and apoptosis under hyperoxic stimulation were detected using 3‑(4,5‑dimethylthi‑azol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay and flow cytometry analysis.

Results: Hyperoxia induced apoptosis in OPCs and decreased their viability. E2 treatment markedly down-regulated the expression of PirB. E2 treatment or PirB silencing markedly decreased hyperoxia-induced apoptosis and increased cell viability in OPCs.

Conclusions: E2 can protect OPCs from hyperoxia-induced apoptosis.

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