Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2016 Jul 13

PMID 27404129

Citations 7

Authors

Christine T Nguyen

Tina I Tsai

Zheng He

Algis J Vingrys

Pei Y Lee

Bang V Bui

Affiliations

Soon will be listed here.

Abstract

The electroretinogram (ERG) and visual evoked potential (VEP) are commonly used to assess the integrity of the visual pathway. The ERG measures the electrical responses of the retina to light stimulation, while the VEP measures the corresponding functional integrity of the visual pathways from the retina to the primary visual cortex following the same light event. The ERG waveform can be broken down into components that reflect responses from different retinal neuronal and glial cell classes. The early components of the VEP waveform represent the integrity of the optic nerve and higher cortical centers. These recordings can be conducted in isolation or together, depending on the application. The methodology described in this paper allows simultaneous assessment of retinal and cortical visual evoked electrophysiology from both eyes and both hemispheres. This is a useful way to more comprehensively assess retinal function and the upstream effects that changes in retinal function can have on visual evoked cortical function.

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References

Kong Y, Van Bergen N, Bui B, Chrysostomou V, Vingrys A, Trounce I . Impact of aging and diet restriction on retinal function during and after acute intraocular pressure injury. Neurobiol Aging. 2012; 33(6):1126.e15-25. DOI: 10.1016/j.neurobiolaging.2011.11.026. View

Yucel Y, Zhang Q, Weinreb R, Kaufman P, Gupta N . Effects of retinal ganglion cell loss on magno-, parvo-, koniocellular pathways in the lateral geniculate nucleus and visual cortex in glaucoma. Prog Retin Eye Res. 2003; 22(4):465-81. DOI: 10.1016/s1350-9462(03)00026-0. View

Tsai T, Bui B, Vingrys A . Effect of acute intraocular pressure challenge on rat retinal and cortical function. Invest Ophthalmol Vis Sci. 2014; 55(2):1067-77. DOI: 10.1167/iovs.13-13003. View

Bui B, Sinclair A, Vingrys A . Electroretinograms of albino and pigmented guinea-pigs (Cavia porcellus). Aust N Z J Ophthalmol. 1998; 26 Suppl 1:S98-100. DOI: 10.1111/j.1442-9071.1998.tb01388.x. View

Cowey A, Franzini C . The retinal origin of uncrossed optic nerve fibres in rats and their role in visual discrimination. Exp Brain Res. 1979; 35(3):443-55. DOI: 10.1007/BF00236763. View

Wostyn P, Audenaert K, De Deyn P . An abnormal high trans-lamina cribrosa pressure difference: a missing link between Alzheimer's disease and glaucoma?. Clin Neurol Neurosurg. 2008; 110(7):753-4. DOI: 10.1016/j.clineuro.2008.05.019. View

Hood D, Birch D . A quantitative measure of the electrical activity of human rod photoreceptors using electroretinography. Vis Neurosci. 1990; 5(4):379-87. DOI: 10.1017/s0952523800000468. View

Charng J, Nguyen C, He Z, Dang T, Vingrys A, Fish R . Conscious wireless electroretinogram and visual evoked potentials in rats. PLoS One. 2013; 8(9):e74172. PMC: 3771909. DOI: 10.1371/journal.pone.0074172. View

Odom J, Bach M, Barber C, Brigell M, Marmor M, Tormene A . Visual evoked potentials standard (2004). Doc Ophthalmol. 2004; 108(2):115-23. DOI: 10.1023/b:doop.0000036790.67234.22. View

10.

Tremblay F, LaRoche R, De Becker I . The electroretinographic diagnosis of the incomplete form of congenital stationary night blindness. Vision Res. 1995; 35(16):2383-93. DOI: 10.1016/0042-6989(95)00006-l. View

11.

Nixon P, Bui B, Armitage J, Vingrys A . The contribution of cone responses to rat electroretinograms. Clin Exp Ophthalmol. 2001; 29(3):193-6. DOI: 10.1046/j.1442-9071.2001.00402.x. View

12.

Bui B, Fortune B . Origin of electroretinogram amplitude growth during light adaptation in pigmented rats. Vis Neurosci. 2006; 23(2):155-67. DOI: 10.1017/S0952523806232024. View

13.

Hudnell H, Boyes W . The comparability of rat and human visual-evoked potentials. Neurosci Biobehav Rev. 1991; 15(1):159-64. DOI: 10.1016/s0149-7634(05)80109-5. View

14.

Weymouth A, Vingrys A . Rodent electroretinography: methods for extraction and interpretation of rod and cone responses. Prog Retin Eye Res. 2007; 27(1):1-44. DOI: 10.1016/j.preteyeres.2007.09.003. View

15.

Ridder 3rd W, Nusinowitz S, Heckenlively J . Causes of cataract development in anesthetized mice. Exp Eye Res. 2002; 75(3):365-70. DOI: 10.1006/exer.2002.2007. View

16.

Robson J, Frishman L . Dissecting the dark-adapted electroretinogram. Doc Ophthalmol. 1999; 95(3-4):187-215. DOI: 10.1023/a:1001891904176. View

17.

Bui B, Vingrys A . Development of receptoral responses in pigmented and albino guinea-pigs (Cavia porcellus). Doc Ophthalmol. 2000; 99(2):151-70. DOI: 10.1023/a:1002721315955. View

18.

Jobling A, Wan R, Gentle A, Bui B, McBrien N . Retinal and choroidal TGF-beta in the tree shrew model of myopia: isoform expression, activation and effects on function. Exp Eye Res. 2008; 88(3):458-66. DOI: 10.1016/j.exer.2008.10.022. View

19.

Birch D, Hood D, Locke K, Hoffman D, Tzekov R . Quantitative electroretinogram measures of phototransduction in cone and rod photoreceptors: normal aging, progression with disease, and test-retest variability. Arch Ophthalmol. 2002; 120(8):1045-51. DOI: 10.1001/archopht.120.8.1045. View

20.

Hetzler B, Berger L . Ketamine-induced modification of photic evoked potentials in the superior colliculus of hooded rats. Neuropharmacology. 1984; 23(4):473-6. DOI: 10.1016/0028-3908(84)90258-2. View