» Articles » PMID: 27403197

JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-β/Smad Signaling

Overview
Date 2016 Jul 13
PMID 27403197
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

JiaWeiDangGui (JWDG) decoction has anti-inflammatory and antifibrotic effects, which is used widely for the treatment of various kidney diseases. In previous studies, we have found that JWDG decoction can reduce the quantity of proteinuria, but the mechanism was unknown. Here, we studied the protective effect of JWDG decoction in adriamycin-induced nephropathy on rat. JWDG decoction, at 10 mL/kg/d, 20 mL/kg/d, and 40 mL/kg/d, was orally administered daily for 12 weeks. Therapeutic effects and mechanisms were further examined. The kidney function related biochemical indexes were measured by automatic biochemistry analyzer. The pathomorphological changes were observed using light and transmission electron microcopies. The proteins expressions of podocin, nephrin, collagen IV, and fibronectin (FN) were examined by immunohistochemical staining, and key proteins involved in TGF-β/Smad signaling were evaluated by RT-PCR and western blotting. Compared with vehicle-treated controls, JWDG decoction decreased the quantity of proteinuria; reduced glomerulosclerotic lesions induced by ADR; and preserved the expression of podocin and nephrin. JWDG decoction also inhibited the expression of the collagen IV, FN, and fibrogenic TGF-β. Further studies revealed that inhibition of renal fibrosis was associated with the blockade of TGF-β/Smad signaling and downregulation of snail expression dose dependently. JWDG decoction prevents proteinuria production, podocyte dysfunction, and kidney injury in adriamycin nephropathy by inhibiting TGF-β/Smad signaling.

Citing Articles

Qigui-Yishen decoction delays renal fibrosis in mice with chronic kidney disease by regulating TM and PAI-1.

Lu X, Wan X Am J Transl Res. 2024; 16(6):2358-2368.

PMID: 39006289 PMC: 11236642. DOI: 10.62347/GHOV4912.


BK Mediates Dysfunction in High Glucose Induced Mesangial Cell Injury via TGF-1/Smad2/3 Signaling Pathways.

Wu Z, Yin W, Sun M, Si Y, Wu X, Chen M Int J Endocrinol. 2020; 2020:3260728.

PMID: 32411221 PMC: 7206888. DOI: 10.1155/2020/3260728.


Effect of Kangxianling Decoction on Expression of TGF-1/Smads and Extracellular Matrix Deposition.

Ji J, He L Evid Based Complement Alternat Med. 2019; 2019:5813549.

PMID: 30713574 PMC: 6332943. DOI: 10.1155/2019/5813549.


Oleanolic acid attenuates TGF-β1-induced epithelial-mesenchymal transition in NRK-52E cells.

He W, Yin J, Cheng X, Lu X, Ni L, Xi Y BMC Complement Altern Med. 2018; 18(1):205.

PMID: 29973206 PMC: 6031099. DOI: 10.1186/s12906-018-2265-y.

References
1.
Choi R, Kim B, Naowaboot J, Lee M, Hyun M, Cho E . Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes. Exp Mol Med. 2011; 43(12):676-83. PMC: 3256295. DOI: 10.3858/emm.2011.43.12.078. View

2.
Huang X, Chung A, Zhou L, Wang X, Lan H . Latent TGF-beta1 protects against crescentic glomerulonephritis. J Am Soc Nephrol. 2008; 19(2):233-42. PMC: 2396747. DOI: 10.1681/ASN.2007040484. View

3.
Wang Y, Wang Y, Tay Y, Harris D . Progressive adriamycin nephropathy in mice: sequence of histologic and immunohistochemical events. Kidney Int. 2000; 58(4):1797-804. DOI: 10.1046/j.1523-1755.2000.00342.x. View

4.
Wu H, Wang Y, Wang Y, Hu M, Zhang G, Knight J . Depletion of gammadelta T cells exacerbates murine adriamycin nephropathy. J Am Soc Nephrol. 2007; 18(4):1180-9. DOI: 10.1681/ASN.2006060622. View

5.
Zhu Y, Huang S, Tan B, Sun J, Whiteman M, Zhu Y . Antioxidants in Chinese herbal medicines: a biochemical perspective. Nat Prod Rep. 2004; 21(4):478-89. DOI: 10.1039/b304821g. View