Effective Cloning of Unmarked DNA Fragments in the Bacillus Subtilis 168 Genome

Overview

Journal Biosci Biotechnol Biochem

Publisher Oxford University Press

Specialties Biochemistry
Biotechnology

Date 2016 Jul 10

PMID 27393265

Citations 11

Authors

M Itaya

Affiliations

Soon will be listed here.

Abstract

The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

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