Platelet Generation in Vivo and in Vitro

Overview

Journal Springerplus

Date 2016 Jul 9

PMID 27390629

Citations 13

Authors

Biao Wang

Jiansheng Zheng

Affiliations

Soon will be listed here.

Abstract

Platelet (PLT) transfusion, which is the primary cell therapy for thrombocytopenia, has been a source of concern in recent years due to its limitations of donor-dependent supply and soaring costs. In vitro platelet generation on an industrial scale is a possible solution requiring exploration. The technology of platelet generation ex vivo has been widely studied across the world, though the mechanisms of physiological thrombopoiesis and platelet biology function in vivo still remain elusive today. Various culture systems have been studied, most of which proved quite inefficient in generating functional platelets ex vivo, so there is still a long way to reach our ultimate goal of generating a fully functional platelet in vitro on an industrial scale. This review integrates the latest research into physiological platelet biogenesis and ex vivo-platelet/megakaryocyte (MK) generation protocols with a focus on the ability to generate PLT/MK in large quantities, summarizes current culture systems based on induced human pluripotent stem cells and adipose-derived stem cells, and discusses significant challenges that must be overcome for these approaches to be perfected.

Citing Articles

Inhibiting the P2Y Receptor in Megakaryocytes and Platelets Suppresses Interferon-Associated Responses.

Sowa M, Sun H, Wang T, Virginio V, Schlamp F, El Bannoudi H JACC Basic Transl Sci. 2024; 9(9):1126-1140.

PMID: 39444926 PMC: 11494392. DOI: 10.1016/j.jacbts.2024.05.014.

An Innovative Inhibitor with a New Chemical Moiety Aimed at Biliverdin IXβ Reductase for Thrombocytopenia and Resilient against Cellular Degradation.

Jung H, Ha J, Dela Cerna M, Burlison J, Choi J, Kim B Pharmaceutics. 2024; 16(9).

PMID: 39339186 PMC: 11435328. DOI: 10.3390/pharmaceutics16091148.

Platelet-Rich Plasma in Dermatology: New Insights on the Cellular Mechanism of Skin Repair and Regeneration.

Manole C, Soare C, Ceafalan L, Voiculescu V Life (Basel). 2024; 14(1).

PMID: 38255655 PMC: 10817627. DOI: 10.3390/life14010040.

Developing a Multichannel Bioreactor with a Collagen Scaffold, ECM, and Cryoprecipitate to Significantly Produce Platelets from Umbilical Cord Blood Stem Cells.

Derakhty Gonbad M, Khoshfetrat A, Movassaghpour A, Sanaat Z, Nozad Charoudeh H Int J Hematol Oncol Stem Cell Res. 2023; 17(4):245-256.

PMID: 38076776 PMC: 10700094. DOI: 10.18502/ijhoscr.v17i4.13916.

Effects of thrombopoietin pre-treatment on peri-liver transplantation thrombocytopenia in a mouse model of cirrhosis with hypersplenism.

Liu Z, Zhang Y, Cui Z, Tong W World J Gastrointest Surg. 2023; 15(10):2115-2122.

PMID: 37969704 PMC: 10642473. DOI: 10.4240/wjgs.v15.i10.2115.

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