Infrared Inhibition of Embryonic Hearts

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2016 Jul 2

PMID 27367249

Citations 18

Authors

Yves T Wang

Andrew M Rollins

Michael W Jenkins

Affiliations

Soon will be listed here.

Abstract

Infrared control is a new technique that uses pulsed infrared lasers to thermally alter electrical activity. Originally developed for nerves, we have applied this technology to embryonic hearts using a quail model, previously demonstrating infrared stimulation and, here, infrared inhibition. Infrared inhibition enables repeatable and reversible block, stopping cardiac contractions for several seconds. Normal beating resumes after the laser is turned off. The block can be spatially specific, affecting propagation on the ventricle or initiation on the atrium. Optical mapping showed that the block affects action potentials and not just calcium or contraction. Increased resting intracellular calcium was observed after a 30-s exposure to the inhibition laser, which likely resulted in reduced mechanical function. Further optimization of the laser illumination should reduce potential damage. Stopping cardiac contractions by disrupting electrical activity with infrared inhibition has the potential to be a powerful tool for studying the developing heart.

Citing Articles

Nanoparticle-based optical interfaces for retinal neuromodulation: a review.

Stoddart P, Begeng J, Tong W, Ibbotson M, Kameneva T Front Cell Neurosci. 2024; 18:1360870.

PMID: 38572073 PMC: 10987880. DOI: 10.3389/fncel.2024.1360870.

Emerging trends in the development of flexible optrode arrays for electrophysiology.

Almasri R, Ladouceur F, Mawad D, Esrafilzadeh D, Firth J, Lehmann T APL Bioeng. 2023; 7(3):031503.

PMID: 37692375 PMC: 10491464. DOI: 10.1063/5.0153753.

Thermal safety considerations for implantable micro-coil design.

Whalen A, Fried S J Neural Eng. 2023; 20(4).

PMID: 37451256 PMC: 10467159. DOI: 10.1088/1741-2552/ace79a.

Computational analysis of multichannel magnetothermal neural stimulation using magnetic resonator array.

Sung K, Jo S, Lee J, Park J, Park Y, Moon J Biomed Eng Lett. 2023; 13(2):209-219.

PMID: 37124115 PMC: 10130299. DOI: 10.1007/s13534-023-00267-x.

Selective Infrared Neural Inhibition Can Be Reproduced by Resistive Heating.

Zhuo J, Weidrick C, Liu Y, Moffitt M, Jansen E, Chiel H Neuromodulation. 2023; 26(8):1757-1771.

PMID: 36707292 PMC: 10366334. DOI: 10.1016/j.neurom.2022.12.004.

References

Kilgore K, Bhadra N . Reversible nerve conduction block using kilohertz frequency alternating current. Neuromodulation. 2013; 17(3):242-54. PMC: 3834124. DOI: 10.1111/ner.12100. View

Wells J, Kao C, Jansen E, Konrad P, Mahadevan-Jansen A . Application of infrared light for in vivo neural stimulation. J Biomed Opt. 2006; 10(6):064003. DOI: 10.1117/1.2121772. View

Hamburger V, HAMILTON H . A series of normal stages in the development of the chick embryo. J Morphol. 2014; 88(1):49-92. View

Jenkins M, Wang Y, Doughman Y, Watanabe M, Cheng Y, Rollins A . Optical pacing of the adult rabbit heart. Biomed Opt Express. 2013; 4(9):1626-35. PMC: 3771833. DOI: 10.1364/BOE.4.001626. View

Orrenius S, Zhivotovsky B, Nicotera P . Regulation of cell death: the calcium-apoptosis link. Nat Rev Mol Cell Biol. 2003; 4(7):552-65. DOI: 10.1038/nrm1150. View

Kitakaze M, Weisman H, Marban E . Contractile dysfunction and ATP depletion after transient calcium overload in perfused ferret hearts. Circulation. 1988; 77(3):685-95. DOI: 10.1161/01.cir.77.3.685. View

Cayce J, Friedman R, Chen G, Jansen E, Mahadevan-Jansen A, Roe A . Infrared neural stimulation of primary visual cortex in non-human primates. Neuroimage. 2013; 84:181-90. PMC: 4120263. DOI: 10.1016/j.neuroimage.2013.08.040. View

Liske H, Towne C, Anikeeva P, Zhao S, Feng G, Deisseroth K . Optical inhibition of motor nerve and muscle activity in vivo. Muscle Nerve. 2013; 47(6):916-21. PMC: 5507203. DOI: 10.1002/mus.23696. View

Wang Y, Gu S, Ma P, Watanabe M, Rollins A, Jenkins M . Optical stimulation enables paced electrophysiological studies in embryonic hearts. Biomed Opt Express. 2014; 5(4):1000-13. PMC: 3985989. DOI: 10.1364/BOE.5.001000. View

10.

Izzo A, Walsh Jr J, Ralph H, Webb J, Bendett M, Wells J . Laser stimulation of auditory neurons: effect of shorter pulse duration and penetration depth. Biophys J. 2008; 94(8):3159-66. PMC: 2275688. DOI: 10.1529/biophysj.107.117150. View

11.

Lumbreras V, Bas E, Gupta C, Rajguru S . Pulsed infrared radiation excites cultured neonatal spiral and vestibular ganglion neurons by modulating mitochondrial calcium cycling. J Neurophysiol. 2014; 112(6):1246-55. PMC: 4137257. DOI: 10.1152/jn.00253.2014. View

12.

Duke A, Jenkins M, Lu H, McManus J, Chiel H, Jansen E . Transient and selective suppression of neural activity with infrared light. Sci Rep. 2013; 3:2600. PMC: 3764437. DOI: 10.1038/srep02600. View

13.

Bhadra N, Kilgore K . Direct current electrical conduction block of peripheral nerve. IEEE Trans Neural Syst Rehabil Eng. 2004; 12(3):313-24. DOI: 10.1109/TNSRE.2004.834205. View

14.

HODGKIN A, Katz B . The effect of temperature on the electrical activity of the giant axon of the squid. J Physiol. 1949; 109(1-2):240-9. PMC: 1392577. DOI: 10.1113/jphysiol.1949.sp004388. View

15.

Fried N, Lagoda G, Scott N, Su L, Burnett A . Noncontact stimulation of the cavernous nerves in the rat prostate using a tunable-wavelength thulium fiber laser. J Endourol. 2008; 22(3):409-13. DOI: 10.1089/end.2008.9996. View

16.

Duke A, Lu H, Jenkins M, Chiel H, Jansen E . Spatial and temporal variability in response to hybrid electro-optical stimulation. J Neural Eng. 2012; 9(3):036003. PMC: 11218549. DOI: 10.1088/1741-2560/9/3/036003. View

17.

Peterson E, Tyler D . Motor neuron activation in peripheral nerves using infrared neural stimulation. J Neural Eng. 2013; 11(1):016001. PMC: 3952042. DOI: 10.1088/1741-2560/11/1/016001. View

18.

Dittami G, Rajguru S, Lasher R, Hitchcock R, Rabbitt R . Intracellular calcium transients evoked by pulsed infrared radiation in neonatal cardiomyocytes. J Physiol. 2011; 589(Pt 6):1295-306. PMC: 3082093. DOI: 10.1113/jphysiol.2010.198804. View

19.

Rajguru S, Richter C, Matic A, Holstein G, Highstein S, Dittami G . Infrared photostimulation of the crista ampullaris. J Physiol. 2011; 589(Pt 6):1283-94. PMC: 3082092. DOI: 10.1113/jphysiol.2010.198333. View

20.

Jenkins M, Duke A, Gu S, Chiel H, Fujioka H, Watanabe M . Optical pacing of the embryonic heart. Nat Photonics. 2011; 4:623-626. PMC: 3059323. DOI: 10.1038/nphoton.2010.166. View