The Effect of Silencing 20E Biosynthesis Relative Genes by Feeding Bacterially Expressed DsRNA on the Larval Development of Chilo Suppressalis

Overview

Journal Sci Rep

Specialty Science

Date 2016 Jun 30

PMID 27352880

Citations 6

Authors

Jian Zhu

Yong-Cheng Dong

Ping Li

Chang-Ying Niu

Affiliations

Soon will be listed here.

Abstract

RNA interference (RNAi) is a robust tool to study gene functions as well as potential for insect pest control. Finding suitable target genes is the key step in the development of an efficient RNAi-mediated pest control technique. Based on the transcriptome of Chilo suppressalis, 24 unigenes which putatively associated with insect hormone biosynthesis were identified. Amongst these, four genes involved in ecdysteroidogenesis i.e., ptth, torso, spook and nm-g were evaluated as candidate targets for function study. The partial cDNA of these four genes were cloned and their bacterially expressed dsRNA were fed to the insects. Results revealed a significant reduction in mRNA abundance of target genes after 3 days. Furthermore, knocked down of these four genes resulted in abnormal phenotypes and high larval mortality. After 15 days, the survival rates of insects in dsspook, dsptth, dstorso, and dsnm-g groups were significantly reduced by 32%, 38%, 56%, and 67% respectively, compared with control. Moreover, about 80% of surviving larvae showed retarded development in dsRNA-treated groups. These results suggest that oral ingestion of bacterially expressed dsRNA in C. suppressalis could silence ptth, torso, spook and nm-g. Oral delivery of bacterially expressed dsRNA provides a simple and potential management scheme against C. suppressalis.

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References

Zhang Y, Zhang S, Kulye M, Wu S, Yu N, Wang J . Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus. J Insect Sci. 2013; 12:91. PMC: 3596932. DOI: 10.1673/031.012.9101. View

Tan A, Palli S . Ecdysone [corrected] receptor isoforms play distinct roles in controlling molting and metamorphosis in the red flour beetle, Tribolium castaneum. Mol Cell Endocrinol. 2008; 291(1-2):42-9. PMC: 2595142. DOI: 10.1016/j.mce.2008.05.006. View

Wang Y, Fan H, Huang H, Xue J, Wu W, Bao Y . Chitin synthase 1 gene and its two alternative splicing variants from two sap-sucking insects, Nilaparvata lugens and Laodelphax striatellus (Hemiptera: Delphacidae). Insect Biochem Mol Biol. 2012; 42(9):637-46. DOI: 10.1016/j.ibmb.2012.04.009. View

Huvenne H, Smagghe G . Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. J Insect Physiol. 2009; 56(3):227-35. DOI: 10.1016/j.jinsphys.2009.10.004. View

Wei Z, Zhang Q, Kang L, Xu W, Denlinger D . Molecular characterization and expression of prothoracicotropic hormone during development and pupal diapause in the cotton bollworm, Helicoverpa armigera. J Insect Physiol. 2005; 51(6):691-700. DOI: 10.1016/j.jinsphys.2005.03.005. View

Niwa R, Sakudoh T, Namiki T, Saida K, Fujimoto Y, Kataoka H . The ecdysteroidogenic P450 Cyp302a1/disembodied from the silkworm, Bombyx mori, is transcriptionally regulated by prothoracicotropic hormone. Insect Mol Biol. 2005; 14(5):563-71. DOI: 10.1111/j.1365-2583.2005.00587.x. View

Xia Y, Zhang Y, Hou X, Li F, Dong S . Large number of putative chemoreception and pheromone biosynthesis genes revealed by analyzing transcriptome from ovipositor-pheromone glands of Chilo suppressalis. Sci Rep. 2015; 5:7888. PMC: 5379011. DOI: 10.1038/srep07888. View

Namiki T, Niwa R, Sakudoh T, Shirai K, Takeuchi H, Kataoka H . Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects. Biochem Biophys Res Commun. 2005; 337(1):367-74. DOI: 10.1016/j.bbrc.2005.09.043. View

Hammond A, Galizi R, Kyrou K, Simoni A, Siniscalchi C, Katsanos D . A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae. Nat Biotechnol. 2015; 34(1):78-83. PMC: 4913862. DOI: 10.1038/nbt.3439. View

10.

Niwa R, Namiki T, Ito K, Shimada-Niwa Y, Kiuchi M, Kawaoka S . Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the 'Black Box' of the ecdysteroid biosynthesis pathway. Development. 2010; 137(12):1991-9. DOI: 10.1242/dev.045641. View

11.

Ono H, Rewitz K, Shinoda T, Itoyama K, Petryk A, Rybczynski R . Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera. Dev Biol. 2006; 298(2):555-70. DOI: 10.1016/j.ydbio.2006.07.023. View

12.

Zhang X, Liu X, Ma J, Zhao J . Silencing of cytochrome P450 CYP6B6 gene of cotton bollworm (Helicoverpa armigera) by RNAi. Bull Entomol Res. 2013; 103(5):584-91. DOI: 10.1017/S0007485313000151. View

13.

Yamanaka N, Rewitz K, OConnor M . Ecdysone control of developmental transitions: lessons from Drosophila research. Annu Rev Entomol. 2012; 58:497-516. PMC: 4060523. DOI: 10.1146/annurev-ento-120811-153608. View

14.

Yamanaka N, Honda N, Osato N, Niwa R, Mizoguchi A, Kataoka H . Differential regulation of ecdysteroidogenic P450 gene expression in the silkworm, Bombyx mori. Biosci Biotechnol Biochem. 2007; 71(11):2808-14. DOI: 10.1271/bbb.70420. View

15.

Iga M, Blais C, Smagghe G . Study on ecdysteroid levels and gene expression of enzymes related to ecdysteroid biosynthesis in the larval testis of Spodoptera littoralis. Arch Insect Biochem Physiol. 2012; 82(1):14-28. DOI: 10.1002/arch.21068. View

16.

Petryk A, Warren J, Marques G, Jarcho M, Gilbert L, Kahler J . Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone. Proc Natl Acad Sci U S A. 2003; 100(24):13773-8. PMC: 283497. DOI: 10.1073/pnas.2336088100. View

17.

Wan P, Jia S, Li N, Fan J, Li G . The putative Halloween gene phantom involved in ecdysteroidogenesis in the white-backed planthopper Sogatella furcifera. Gene. 2014; 548(1):112-8. DOI: 10.1016/j.gene.2014.07.023. View

18.

Rewitz K, Rybczynski R, Warren J, Gilbert L . Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta. Insect Biochem Mol Biol. 2006; 36(3):188-99. DOI: 10.1016/j.ibmb.2005.12.002. View

19.

Rewitz K, Larsen M, Lobner-Olesen A, Rybczynski R, OConnor M, Gilbert L . A phosphoproteomics approach to elucidate neuropeptide signal transduction controlling insect metamorphosis. Insect Biochem Mol Biol. 2009; 39(7):475-83. DOI: 10.1016/j.ibmb.2009.04.005. View

20.

Jia S, Wan P, Zhou L, Mu L, Li G . Knockdown of a putative Halloween gene Shade reveals its role in ecdysteroidogenesis in the small brown planthopper Laodelphax striatellus. Gene. 2013; 531(2):168-74. DOI: 10.1016/j.gene.2013.09.034. View