Biochip for the Detection of Bacillus Anthracis Lethal Factor and Therapeutic Agents Against Anthrax Toxins

Overview

Journal Membranes (Basel)

Date 2016 Jun 28

PMID 27348008

Citations 2

Authors

Vitalii Silin

John J Kasianowicz

Ariel Michelman-Ribeiro

Rekha G Panchal

Sina Bavari

Joseph W F Robertson

Affiliations

Soon will be listed here.

Abstract

Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels that are both the recognition element and electrochemical transducer. We characterize the sensor and its properties with electrochemical impedance spectroscopy and surface plasmon resonance. The sensor shows a sensitivity similar to ELISA and can also be used to rapidly screen for molecules that bind to the toxins and potentially inhibit their lethal effects.

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