Variation in Copy Number of the 28S RDNA of Aspergillus Fumigatus Measured by Droplet Digital PCR and Analog Quantitative Real-time PCR

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2016 Jun 19

PMID 27316653

Citations 10

Authors

Alexandre Alanio

Aude Sturny-Leclere

Marion Benabou

Nicolas Guigue

Stephane Bretagne

Affiliations

Soon will be listed here.

Abstract

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.

Citing Articles

Are short-read amplicons suitable for the prediction of microbiome functional potential? A critical perspective.

Heidrich V, Beule L Imeta. 2024; 1(3):e38.

PMID: 38868716 PMC: 10989910. DOI: 10.1002/imt2.38.

Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study.

Mbangiwa T, Sturny-Leclere A, Lechiile K, Kajanga C, Boyer-Chammard T, Hoving J Lancet Microbe. 2024; 5(3):e261-e271.

PMID: 38342110 PMC: 10914677. DOI: 10.1016/S2666-5247(23)00362-2.

An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections.

Jenks J, White P, Kidd S, Goshia T, Fraley S, Hoenigl M Expert Rev Mol Diagn. 2023; 23(12):1135-1152.

PMID: 37801397 PMC: 10842420. DOI: 10.1080/14737159.2023.2267977.

Development and clinical validation of a droplet digital PCR assay for detecting Acinetobacter baumannii and Klebsiella pneumoniae in patients with suspected bloodstream infections.

Zheng Y, Jin J, Shao Z, Liu J, Zhang R, Sun R Microbiologyopen. 2021; 10(6):e1247.

PMID: 34964298 PMC: 8594765. DOI: 10.1002/mbo3.1247.

Recent Advances and Novel Approaches in Laboratory-Based Diagnostic Mycology.

White L, Price J J Fungi (Basel). 2021; 7(1).

PMID: 33440757 PMC: 7827937. DOI: 10.3390/jof7010041.