Hematological, Biochemical, and Serological Findings in Healthy Canine Blood Donors After the Administration of CaniLeish® Vaccine

Overview

Journal Vet Med Int

Publisher Wiley

Specialty Veterinary Medicine

Date 2016 Jun 18

PMID 27313949

Citations 5

Authors

Chiara Starita

Alessandra Gavazza

George Lubas

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to evaluate hematological, biochemical, and serological findings in healthy canine blood donors after the administration of CaniLeish® vaccine. Twenty-seven client-owned dogs were included in the study and arranged into 3 groups according to the vaccination stage. Complete blood count (CBC) with blood smear examination, serum biochemical profile (SBP), serum protein electrophoresis (SPE), and serological tests for L. infantum were performed at different times. Additionally, in a subgroup of dogs IgA, IgM, and IgG were quantified. No statistical significance for CBC and SBP was found. In 10.7% of cases slight hyperproteinemia occurred. In SPE absolute values β-1-globulins (Group 2 and Group 2-3) and β-2-globulins (Group 3) were found modified (P < 0.05). IgG values were statistically different (P < 0.05) 6-8 months after the third immunisation (Group 2) and IgM and IgG values were statistically different after 2 months (Group 3). IFAT positive samples were 20.8% (Group 1), 15.0% (Group 2), and 52.8% (Group 3). Speed Leish K™ tests were always negative. The modifications found were probably attributed to the development of immune or inflammatory response due to the vaccine. Administration of CaniLeish vaccine in canine blood donors could be a safe practice and did not affect their health status.

Citing Articles

Central and Effector Memory Human CD4+ and CD8+ T Cells during Cutaneous Leishmaniasis and after In Vitro Stimulation with Leishmania (Viannia) braziliensis Epitopes.

de Oliveira B, da Silva A, Kaique de Andrade Cavalcante M, de Brito M, Accioly Brelaz de Castro M, de Medeiros V Vaccines (Basel). 2023; 11(1).

PMID: 36680003 PMC: 9861845. DOI: 10.3390/vaccines11010158.

Vaccines for leishmaniasis and the implications of their development for American tegumentary leishmaniasis.

de Oliveira B, Duthie M, Alves Pereira V Hum Vaccin Immunother. 2019; 16(4):919-930.

PMID: 31634036 PMC: 7227727. DOI: 10.1080/21645515.2019.1678998.

Vaccines Entered in Clinical Trials: A Review of Literature.

Moafi M, Rezvan H, Sherkat R, Taleban R Int J Prev Med. 2019; 10:95.

PMID: 31360342 PMC: 6592111. DOI: 10.4103/ijpvm.IJPVM_116_18.

Safety Analysis of Vaccine Used in a Randomized Canine Vaccine/Immunotherapy Trial.

Toepp A, Larson M, Grinnage-Pulley T, Bennett C, Anderson M, Parrish M Am J Trop Med Hyg. 2018; 98(5):1332-1338.

PMID: 29512486 PMC: 5953386. DOI: 10.4269/ajtmh.17-0888.

A Chimera Containing CD4+ and CD8+ T-Cell Epitopes of the Nucleoside Hydrolase (NH36) Optimizes Cross-Protection against Infection.

Alves-Silva M, Nico D, Morrot A, Palatnik M, Palatnik-de-Sousa C Front Immunol. 2017; 8:100.

PMID: 28280494 PMC: 5322207. DOI: 10.3389/fimmu.2017.00100.

References

Owens S, Oakley D, Marryott K, Hatchett W, Walton R, Nolan T . Transmission of visceral leishmaniasis through blood transfusions from infected English foxhounds to anemic dogs. J Am Vet Med Assoc. 2001; 219(8):1076-83. DOI: 10.2460/javma.2001.219.1076. View

de Freitas E, Melo M, da Costa-Val A, Michalick M . Transmission of Leishmania infantum via blood transfusion in dogs: potential for infection and importance of clinical factors. Vet Parasitol. 2006; 137(1-2):159-67. DOI: 10.1016/j.vetpar.2005.12.011. View

Tabar M, Roura X, Francino O, Altet L, Ruiz de Gopegui R . Detection of Leishmania infantum by real-time PCR in a canine blood bank. J Small Anim Pract. 2008; 49(7):325-8. DOI: 10.1111/j.1748-5827.2008.00542.x. View

Paltrinieri S, Solano-Gallego L, Fondati A, Lubas G, Gradoni L, Castagnaro M . Guidelines for diagnosis and clinical classification of leishmaniasis in dogs. J Am Vet Med Assoc. 2010; 236(11):1184-91. DOI: 10.2460/javma.236.11.1184. View

Solano-Gallego L, Miro G, Koutinas A, Cardoso L, Pennisi M, Ferrer L . LeishVet guidelines for the practical management of canine leishmaniosis. Parasit Vectors. 2011; 4:86. PMC: 3125381. DOI: 10.1186/1756-3305-4-86. View

Maroli M, Gradoni L, Oliva G, Castagnaro M, Crotti A, Lubas G . Guidelines for prevention of leishmaniasis in dogs. J Am Vet Med Assoc. 2010; 236(11):1200-6. DOI: 10.2460/javma.236.11.1200. View

Martin V, Vouldoukis I, Moreno J, McGahie D, Gueguen S, Cuisinier A . The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later. Vet Res. 2014; 45:69. PMC: 4086268. DOI: 10.1186/1297-9716-45-69. View

Tvarijonaviciute A, Martinez-Subiela S, Caldin M, Tecles F, Ceron J . Evaluation of automated assays for immunoglobulin G, M, and A measurements in dog and cat serum. Vet Clin Pathol. 2013; 42(3):270-80. DOI: 10.1111/vcp.12069. View

Oliva G, Nieto J, Manzillo V, Cappiello S, Fiorentino E, Di Muccio T . A randomised, double-blind, controlled efficacy trial of the LiESP/QA-21 vaccine in naïve dogs exposed to two leishmania infantum transmission seasons. PLoS Negl Trop Dis. 2014; 8(10):e3213. PMC: 4191955. DOI: 10.1371/journal.pntd.0003213. View

10.

Otranto D, Dantas-Torres F . The prevention of canine leishmaniasis and its impact on public health. Trends Parasitol. 2013; 29(7):339-45. DOI: 10.1016/j.pt.2013.05.003. View

11.

Ebani V, Bertelloni F, Torracca B, Cerri D . Serological survey of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Ehrlichia canis infections in rural and urban dogs in Central Italy. Ann Agric Environ Med. 2014; 21(4):671-5. DOI: 10.5604/12321966.1129912. View

12.

Mancianti F, Meciani N . Specific serodiagnosis of canine leishmaniasis by indirect immunofluorescence, indirect hemagglutination, and counterimmunoelectrophoresis. Am J Vet Res. 1988; 49(8):1409-11. View

13.

Solano-Gallego L, Villanueva-Saz S, Carbonell M, Trotta M, Furlanello T, Natale A . Serological diagnosis of canine leishmaniosis: comparison of three commercial ELISA tests (Leiscan, ID Screen and Leishmania 96), a rapid test (Speed Leish K) and an in-house IFAT. Parasit Vectors. 2014; 7:111. PMC: 3994334. DOI: 10.1186/1756-3305-7-111. View

14.

Bongiorno G, Paparcone R, Manzillo V, Oliva G, Cuisinier A, Gradoni L . Vaccination with LiESP/QA-21 (CaniLeish®) reduces the intensity of infection in Phlebotomus perniciosus fed on Leishmania infantum infected dogs--a preliminary xenodiagnosis study. Vet Parasitol. 2013; 197(3-4):691-5. DOI: 10.1016/j.vetpar.2013.05.008. View

15.

Moreno J, Vouldoukis I, Schreiber P, Martin V, McGahie D, Gueguen S . Primary vaccination with the LiESP/QA-21 vaccine (CaniLeish) produces a cell-mediated immune response which is still present 1 year later. Vet Immunol Immunopathol. 2014; 158(3-4):199-207. DOI: 10.1016/j.vetimm.2014.01.011. View

16.

Solano-Gallego L, Koutinas A, Miro G, Cardoso L, Pennisi M, Ferrer L . Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis. Vet Parasitol. 2009; 165(1-2):1-18. DOI: 10.1016/j.vetpar.2009.05.022. View