Enhancing Cellulase Production by Overexpression of Xylanase Regulator Protein Gene, XlnR, in Talaromyces Cellulolyticus Cellulase Hyperproducing Mutant Strain

Overview

Journal Biosci Biotechnol Biochem

Publisher Oxford University Press

Specialties Biochemistry
Biotechnology

Date 2016 Jun 17

PMID 27309759

Citations 4

Authors

Naoyuki Okuda

Tatsuya Fujii

Hiroyuki Inoue

Kazuhiko Ishikawa

Tamotsu Hoshino

Affiliations

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Abstract

We obtained strains with the xylanase regulator gene, xlnR, overexpressed (HXlnR) and disrupted (DXlnR) derived from Talaromyces cellulolyticus strain C-1, which is a cellulase hyperproducing mutant. Filter paper degrading enzyme activity and cellobiohydrolase I gene expression was the highest in HXlnR, followed by C-1 and DXlnR. These results indicate that the enhancement of cellulase productivity was succeeded by xlnR overexpression.

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