Revealing the Combined Effects of Lactulose and Probiotic Enterococci on the Swine Faecal Microbiota Using 454 Pyrosequencing

Overview

Journal Microb Biotechnol

Specialties Biotechnology
Microbiology

Date 2016 Jun 17

PMID 27305897

Citations 25

Authors

Jong Pyo Chae

Edward Alain B Pajarillo

Ju Kyoung Oh

Heebal Kim

Dae-Kyung Kang

Affiliations

Soon will be listed here.

Abstract

Demand for the development of non-antibiotic growth promoters in animal production has increased in recent years. This report compared the faecal microbiota of weaned piglets under the administration of a basal diet (CON) or that containing prebiotic lactulose (LAC), probiotic Enterococcus faecium NCIMB 11181 (PRO) or their synbiotic combination (SYN). At the phylum level, the Firmicutes to Bacteroidetes ratio increased in the treatment groups compared with the CON group, and the lowest proportion of Proteobacteria was observed in the LAC group. At the family level, Enterobacteriaceae decreased in all treatments; more than a 10-fold reduction was observed in the LAC (0.99%) group compared with the CON group. At the genus level, the highest Oscillibacter proportion was detected in PRO, the highest Clostridium in LAC and the highest Lactobacillus in SYN; the abundance of Escherichia was lowest in the LAC group. Clustering in the discriminant analysis of principal components revealed distinct separation of the feeding groups (CON, LAC, PRO and SYN), showing different microbial compositions according to different feed additives or their combination. These results suggest that individual materials and their combination have unique actions and independent mechanisms for changes in the distal gut microbiota.

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References

Yan H, Potu R, Lu H, de Almeida V, Stewart T, Ragland D . Dietary fat content and fiber type modulate hind gut microbial community and metabolic markers in the pig. PLoS One. 2013; 8(4):e59581. PMC: 3616062. DOI: 10.1371/journal.pone.0059581. View

Zheng W, Hou Y, Su Y, Yao W . Lactulose promotes equol production and changes the microbial community during in vitro fermentation of daidzein by fecal inocula of sows. Anaerobe. 2013; 25:47-52. DOI: 10.1016/j.anaerobe.2013.11.011. View

Umu O, Frank J, Fangel J, Oostindjer M, Da Silva C, Bolhuis E . Resistant starch diet induces change in the swine microbiome and a predominance of beneficial bacterial populations. Microbiome. 2015; 3:16. PMC: 4405844. DOI: 10.1186/s40168-015-0078-5. View

Bajaj J, Ridlon J, Hylemon P, Thacker L, Heuman D, Smith S . Linkage of gut microbiome with cognition in hepatic encephalopathy. Am J Physiol Gastrointest Liver Physiol. 2011; 302(1):G168-75. PMC: 3345956. DOI: 10.1152/ajpgi.00190.2011. View

Jeon Y, Chun J, Kim B . Identification of household bacterial community and analysis of species shared with human microbiome. Curr Microbiol. 2013; 67(5):557-63. PMC: 3790245. DOI: 10.1007/s00284-013-0401-y. View

Hemarajata P, Versalovic J . Effects of probiotics on gut microbiota: mechanisms of intestinal immunomodulation and neuromodulation. Therap Adv Gastroenterol. 2013; 6(1):39-51. PMC: 3539293. DOI: 10.1177/1756283X12459294. View

Konstantinov S, Awati A, Smidt H, Williams B, Akkermans A, de Vos W . Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets. Appl Environ Microbiol. 2004; 70(7):3821-30. PMC: 444839. DOI: 10.1128/AEM.70.7.3821-3830.2004. View

Kim H, Isaacson R . The pig gut microbial diversity: Understanding the pig gut microbial ecology through the next generation high throughput sequencing. Vet Microbiol. 2015; 177(3-4):242-51. DOI: 10.1016/j.vetmic.2015.03.014. View

Lalles J, Bosi P, Smidt H, Stokes C . Nutritional management of gut health in pigs around weaning. Proc Nutr Soc. 2007; 66(2):260-8. DOI: 10.1017/S0029665107005484. View

10.

Pajarillo E, Chae J, Balolong M, Kim H, Seo K, Kang D . Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines. J Microbiol. 2014; 52(8):646-51. DOI: 10.1007/s12275-014-4270-2. View

11.

OConnor E, OHerlihy E, OToole P . Gut microbiota in older subjects: variation, health consequences and dietary intervention prospects. Proc Nutr Soc. 2014; 73(4):441-51. DOI: 10.1017/S0029665114000597. View

12.

Hooda S, Vester Boler B, Serao M, Brulc J, Staeger M, Boileau T . 454 pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber. J Nutr. 2012; 142(7):1259-65. DOI: 10.3945/jn.112.158766. View

13.

Unno T, Kim J, Guevarra R, Nguyen S . Effects of antibiotic growth promoter and characterization of ecological succession in Swine gut microbiota. J Microbiol Biotechnol. 2014; 25(4):431-8. DOI: 10.4014/jmb.1408.08063. View

14.

Bomba L, Minuti A, Moisa S, Trevisi E, Eufemi E, Lizier M . Gut response induced by weaning in piglet features marked changes in immune and inflammatory response. Funct Integr Genomics. 2014; 14(4):657-71. DOI: 10.1007/s10142-014-0396-x. View

15.

Guerra-Ordaz A, Gonzalez-Ortiz G, La Ragione R, Woodward M, Collins J, Perez J . Lactulose and Lactobacillus plantarum, a potential complementary synbiotic to control postweaning colibacillosis in piglets. Appl Environ Microbiol. 2014; 80(16):4879-86. PMC: 4135760. DOI: 10.1128/AEM.00770-14. View

16.

Li W, Godzik A . Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006; 22(13):1658-9. DOI: 10.1093/bioinformatics/btl158. View

17.

Kim H, Borewicz K, White B, Singer R, Sreevatsan S, Tu Z . Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs. Vet Microbiol. 2011; 153(1-2):124-33. DOI: 10.1016/j.vetmic.2011.05.021. View

18.

Lee J, Awji E, Lee S, Tassew D, Park Y, Park K . Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli. J Anim Sci. 2012; 90(11):3709-17. DOI: 10.2527/jas.2011-4434. View

19.

Mao B, Li D, Zhao J, Liu X, Gu Z, Chen Y . In vitro fermentation of lactulose by human gut bacteria. J Agric Food Chem. 2014; 62(45):10970-7. DOI: 10.1021/jf503484d. View

20.

Ito Y, Moriwaki H, Muto Y, Kato N, Watanabe K, Ueno K . Effect of lactulose on short-chain fatty acids and lactate production and on the growth of faecal flora, with special reference to Clostridium difficile. J Med Microbiol. 1997; 46(1):80-4. DOI: 10.1099/00222615-46-1-80. View