Structure of an ABC Transporter Solute-binding Protein Specific for the Amino Sugars Glucosamine and Galactosamine

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2016 Jun 16

PMID 27303900

Citations 2

Authors

Umesh Yadava

Matthew W Vetting

Nawar Al Obaidi

Michael S Carter

John A Gerlt

Steven C Almo

Affiliations

Soon will be listed here.

Abstract

The uptake of exogenous solutes by prokaryotes is mediated by transport systems embedded in the plasma membrane. In many cases, a solute-binding protein (SBP) is utilized to bind ligands with high affinity and deliver them to the membrane-bound components responsible for translocation into the cytoplasm. In the present study, Avi_5305, an Agrobacterium vitis SBP belonging to Pfam13407, was screened by differential scanning fluorimetry (DSF) and found to be stabilized by D-glucosamine and D-galactosamine. Avi_5305 is the first protein from Pfam13407 shown to be specific for amino sugars, and co-crystallization resulted in structures of Avi_5305 bound to D-glucosamine and D-galactosamine. Typical of Pfam13407, Avi_5305 consists of two α/β domains linked through a hinge region, with the ligand-binding site located in a cleft between the two domains. Comparisons with Escherichia coli ribose-binding protein suggest that a cation-π interaction with Tyr168 provides the specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose.

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