Antioxidant, Anti-inflammatory and Anticancer Activities of the Medicinal Halophyte Reaumuria Vermiculata

Overview

Journal EXCLI J

Specialty Biology

Date 2016 Jun 15

PMID 27298615

Citations 9

Authors

Manel Karker

Hanen Falleh

Kamel Msaada

Abderrazak Smaoui

Chedly Abdelly

Jean Legault

Riadh Ksouri

Affiliations

Soon will be listed here.

Abstract

Reaumuria vermiculata is a xero-halophytic specie widely distributed in the south of Tunisia. In the current study, antioxidant, anti-inflammatory and anticancer activities of Reaumuria vermiculata shoot extracts as well as its phenolic compounds were investigated in different solvent extracts (hexane, dichloromethane, methanol and water). Results showed a strong antioxidant activity, using the ORAC method and a cell based-assay, in methanol extract as well as an important phenolic composition (117.12 mg GAE/g). Hexane and dichloromethane proved an interesting anticancer activity against A-549 lung carcinoma cells, with IC50 values of 17 and 23 µg/ml, respectively. Besides, dichloromethane extract displayed the utmost anti-inflammatory activity, inhibiting NO release over 100 % at 80 µg/ml in LPS-stimulated RAW 264.7. Taken together, these finding suggest that R. vermiculata exhibited an interesting biological activities which may be related to the phenolic composition of this plant. Moreover, the identification of phenolic compounds in R. vermiculata dichloromethane extract using RP-HPLC revealed that myricetin was the major molecule. These results allow us to propose R. vermiculata as a valuable source for bioactive and natural compounds exhibiting interesting biological capacities.

Citing Articles

LC-QTOF/MS-Based Profiling of the Phytochemicals in Ice Plant () and Their Bioactivities.

Oh M, Han A, Lee J, Choi S, Choi J, Song N Foods. 2024; 13(12).

PMID: 38928762 PMC: 11202690. DOI: 10.3390/foods13121820.

Salt-Tolerant Plants, Halophytes, as Renewable Natural Resources for Cancer Prevention and Treatment: Roles of Phenolics and Flavonoids in Immunomodulation and Suppression of Oxidative Stress towards Cancer Management.

Mohammed H, Emwas A, Khan R Int J Mol Sci. 2023; 24(6).

PMID: 36982245 PMC: 10048981. DOI: 10.3390/ijms24065171.

Bioavailability of Phenolic Compounds in Californian-Style Table Olives with Tunisian Aqueous Olive Leaf Extracts.

Mechi D, Baccouri B, Martin-Vertedor D, Abaza L Molecules. 2023; 28(2).

PMID: 36677765 PMC: 9866685. DOI: 10.3390/molecules28020707.

Herb and Spices in Colorectal Cancer Prevention and Treatment: A Narrative Review.

Hossain M, Kader M, Goh K, Islam M, Khan M, Rashid M Front Pharmacol. 2022; 13:865801.

PMID: 35846992 PMC: 9280164. DOI: 10.3389/fphar.2022.865801.

Antioxidant Properties, Phytoactive Compounds and Potential Protective Action of Flowers Against Combined Gastro-Intestinal Ulcer and Diarrhea Experimentally Induced in Rat.

Jedidi S, Selmi H, Aloui F, Rtibi K, Sammari H, Abbes C Dose Response. 2022; 20(2):15593258221102313.

PMID: 35602586 PMC: 9122491. DOI: 10.1177/15593258221102313.

References

Andlauer W, Furst P . Special characteristics of non-nutrient food constituents of plants--phytochemicals. Introductory lecture. Int J Vitam Nutr Res. 2003; 73(2):55-62. DOI: 10.1024/0300-9831.73.2.55. View

Stevigny C, Bailly C, Quetin-Leclercq J . Cytotoxic and antitumor potentialities of aporphinoid alkaloids. Curr Med Chem Anticancer Agents. 2005; 5(2):173-82. DOI: 10.2174/1568011053174864. View

Legault J, Dahl W, Debiton E, Pichette A, Madelmont J . Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. Planta Med. 2003; 69(5):402-7. DOI: 10.1055/s-2003-39695. View

Ksouri W, Medini F, Mkadmini K, Legault J, Magne C, Abdelly C . LC-ESI-TOF-MS identification of bioactive secondary metabolites involved in the antioxidant, anti-inflammatory and anticancer activities of the edible halophyte Zygophyllum album Desf. Food Chem. 2013; 139(1-4):1073-80. DOI: 10.1016/j.foodchem.2013.01.047. View

Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K . Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food Chem Toxicol. 2009; 47(8):2083-91. DOI: 10.1016/j.fct.2009.05.040. View

Dufour D, Pichette A, Mshvildadze V, Bradette-Hebert M, Lavoie S, Longtin A . Antioxidant, anti-inflammatory and anticancer activities of methanolic extracts from Ledum groenlandicum Retzius. J Ethnopharmacol. 2006; 111(1):22-8. DOI: 10.1016/j.jep.2006.10.021. View

Vuorela S, Kreander K, Karonen M, Nieminen R, Hamalainen M, Galkin A . Preclinical evaluation of rapeseed, raspberry, and pine bark phenolics for health related effects. J Agric Food Chem. 2005; 53(15):5922-31. DOI: 10.1021/jf050554r. View

Hanen F, Riadh K, Samia O, Sylvain G, Christian M, Chedly A . Interspecific variability of antioxidant activities and phenolic composition in Mesembryanthemum genus. Food Chem Toxicol. 2009; 47(9):2308-13. DOI: 10.1016/j.fct.2009.06.025. View

Luo W, Gong X, Chang A . An ER membrane protein, Sop4, facilitates ER export of the yeast plasma membrane [H+]ATPase, Pma1. Traffic. 2002; 3(10):730-9. DOI: 10.1034/j.1600-0854.2002.31005.x. View

10.

Dewanto V, Wu X, Adom K, Liu R . Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem. 2002; 50(10):3010-4. DOI: 10.1021/jf0115589. View

11.

Yangthong M, Hutadilok-Towatana N, Phromkunthong W . Antioxidant activities of four edible seaweeds from the southern coast of Thailand. Plant Foods Hum Nutr. 2009; 64(3):218-23. DOI: 10.1007/s11130-009-0127-y. View

12.

Wang S, Kuo Y, Chang H, Kang P, Tsay H, Lin K . Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata. J Agric Food Chem. 2002; 50(7):1859-65. DOI: 10.1021/jf0113575. View

13.

OBrien J, Wilson I, Orton T, Pognan F . Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000; 267(17):5421-6. DOI: 10.1046/j.1432-1327.2000.01606.x. View

14.

Sehrawat A, Sultana S . Evaluation of possible mechanisms of protective role of Tamarix gallica against DEN initiated and 2-AAF promoted hepatocarcinogenesis in male Wistar rats. Life Sci. 2006; 79(15):1456-65. DOI: 10.1016/j.lfs.2006.04.009. View

15.

Ou B, Prior R . Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem. 2001; 49(10):4619-26. DOI: 10.1021/jf010586o. View

16.

Nawwar M, Ayoub N, El-Rai M, Bassyouny F, Mostafa E, Al-Abd A . Cytotoxic ellagitannins from Reaumuria vermiculata. Fitoterapia. 2012; 83(7):1256-66. DOI: 10.1016/j.fitote.2012.06.007. View

17.

van der Watt E, Pretorius J . Purification and identification of active antibacterial components in Carpobrotus edulis L. J Ethnopharmacol. 2001; 76(1):87-91. DOI: 10.1016/s0378-8741(01)00197-0. View

18.

Wang Y, Chen P, Tang C, Wang Y, Li Y, Zhang H . Antinociceptive and anti-inflammatory activities of extract and two isolated flavonoids of Carthamus tinctorius L. J Ethnopharmacol. 2013; 151(2):944-50. DOI: 10.1016/j.jep.2013.12.003. View

19.

Jiang F, Dusting G . Natural phenolic compounds as cardiovascular therapeutics: potential role of their antiinflammatory effects. Curr Vasc Pharmacol. 2004; 1(2):135-56. DOI: 10.2174/1570161033476736. View

20.

Green S, Meltzer M, Hibbs Jr J, Nacy C . Activated macrophages destroy intracellular Leishmania major amastigotes by an L-arginine-dependent killing mechanism. J Immunol. 1990; 144(1):278-83. View