A Click Chemistry-based MicroRNA Maturation Assay Optimized for High-throughput Screening

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2016 Jun 11

PMID 27284591

Citations 20

Authors

Daniel A Lorenz

Amanda L Garner

Affiliations

Soon will be listed here.

Abstract

Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels-Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.

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