Graphene Oxide-based Electrochemical Label-free Detection of Glycoproteins Down to AM Level Using a Lectin Biosensor

Overview

Journal Analyst

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2016 Jun 10

PMID 27277703

Citations 6

Authors

L Klukova

J Filip

S Belicky

A Vikartovska

J Tkac

Affiliations

Soon will be listed here.

Abstract

A label-free ultrasensitive impedimetric biosensor with lectin immobilised on graphene oxide (GO) for the detection of glycoproteins from 1 aM is shown here. This is the first time a functional lectin biosensor with lectin directly immobilised on a graphene-based interface without any polymer modifier has been described. The study also shows that hydrophilic oxidative debris present on GO has a beneficial effect on the sensitivity of (8.46 ± 0.20)% per decade for the lectin biosensor compared to the sensitivity of (4.52 ± 0.23)% per decade for the lectin biosensor built up from GO with the oxidative debris washed out.

Citing Articles

Deciphering disease through glycan codes: leveraging lectin microarrays for clinical insights.

Yang H, Lin Z, Wu B, Xu J, Tao S, Zhou S Acta Biochim Biophys Sin (Shanghai). 2024; 56(8):1145-1155.

PMID: 39099413 PMC: 11399442. DOI: 10.3724/abbs.2024123.

Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis.

Fu L, Zheng Y, Li X, Liu X, Lin C, Karimi-Maleh H Molecules. 2023; 28(18).

PMID: 37764496 PMC: 10536827. DOI: 10.3390/molecules28186719.

Research advances and prospects of legume lectins.

Katoch R, Tripathi A J Biosci. 2021; 46.

PMID: 34815374 PMC: 8608583.

Combating Actions of Green 2D-Materials on Gram Positive and Negative Bacteria and Enveloped Viruses.

Singh M, Zannella C, Folliero V, Di Girolamo R, Bajardi F, Chianese A Front Bioeng Biotechnol. 2020; 8:569967.

PMID: 33117781 PMC: 7549698. DOI: 10.3389/fbioe.2020.569967.

A Graphene-Based Glycan Biosensor for Electrochemical Label-Free Detection of a Tumor-Associated Antibody.

Kveton F, Blsakova A, Lorencova L, Jerigova M, Velic D, Blixt O Sensors (Basel). 2019; 19(24).

PMID: 31818011 PMC: 6960651. DOI: 10.3390/s19245409.

References

Huang J, Zhang L, Liang R, Qiu J . "On-off" switchable electrochemical affinity nanobiosensor based on graphene oxide for ultrasensitive glucose sensing. Biosens Bioelectron. 2012; 41:430-5. DOI: 10.1016/j.bios.2012.09.007. View

Klukova L, Bertok T, Petrikova M, Sediva A, Mislovicova D, Katrlik J . Glycoprofiling as a novel tool in serological assays of systemic sclerosis: a comparative study with three bioanalytical methods. Anal Chim Acta. 2014; 853:555-562. PMC: 4883648. DOI: 10.1016/j.aca.2014.10.029. View

Bertok T, Sediva A, Filip J, Ilcikova M, Kasak P, Velic D . Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum. Langmuir. 2015; 31(25):7148-57. PMC: 4489201. DOI: 10.1021/acs.langmuir.5b00944. View

He X, Zhu B, Zang Y, Li J, Chen G, Tian H . Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes. Chem Sci. 2017; 6(3):1996-2001. PMC: 5496384. DOI: 10.1039/c4sc03614j. View

Rabinovich G, Toscano M . Turning 'sweet' on immunity: galectin-glycan interactions in immune tolerance and inflammation. Nat Rev Immunol. 2009; 9(5):338-52. DOI: 10.1038/nri2536. View

Song Y, Liu H, Tan H, Xu F, Jia J, Zhang L . pH-switchable electrochemical sensing platform based on chitosan-reduced graphene oxide/concanavalin a layer for assay of glucose and urea. Anal Chem. 2014; 86(4):1980-7. DOI: 10.1021/ac402742m. View

Novoselov K, Geim A, Morozov S, Jiang D, Zhang Y, Dubonos S . Electric field effect in atomically thin carbon films. Science. 2004; 306(5696):666-9. DOI: 10.1126/science.1102896. View

Tang Q, Zhou Z, Chen Z . Graphene-related nanomaterials: tuning properties by functionalization. Nanoscale. 2013; 5(11):4541-83. DOI: 10.1039/c3nr33218g. View

Pumera M . Graphene-based nanomaterials and their electrochemistry. Chem Soc Rev. 2010; 39(11):4146-57. DOI: 10.1039/c002690p. View

10.

Fuster M, Esko J . The sweet and sour of cancer: glycans as novel therapeutic targets. Nat Rev Cancer. 2005; 5(7):526-42. DOI: 10.1038/nrc1649. View

11.

Liu J, Wang J, Wang T, Li D, Xi F, Wang J . Three-dimensional electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on monolithic and macroporous graphene foam. Biosens Bioelectron. 2014; 65:281-6. DOI: 10.1016/j.bios.2014.10.016. View

12.

Georgakilas V, Otyepka M, Bourlinos A, Chandra V, Kim N, Kemp K . Functionalization of graphene: covalent and non-covalent approaches, derivatives and applications. Chem Rev. 2012; 112(11):6156-214. DOI: 10.1021/cr3000412. View

13.

Geim A . Graphene: status and prospects. Science. 2009; 324(5934):1530-4. DOI: 10.1126/science.1158877. View

14.

Novoselov K, Falko V, Colombo L, Gellert P, Schwab M, Kim K . A roadmap for graphene. Nature. 2012; 490(7419):192-200. DOI: 10.1038/nature11458. View

15.

Palecek E, Tkac J, Bartosik M, Bertok T, Ostatna V, Palecek J . Electrochemistry of nonconjugated proteins and glycoproteins. Toward sensors for biomedicine and glycomics. Chem Rev. 2015; 115(5):2045-108. PMC: 4360380. DOI: 10.1021/cr500279h. View

16.

Bonanni A, Ambrosi A, Chua C, Pumera M . Oxidation debris in graphene oxide is responsible for its inherent electroactivity. ACS Nano. 2014; 8(5):4197-204. DOI: 10.1021/nn404255q. View

17.

Liu Y, Dong X, Chen P . Biological and chemical sensors based on graphene materials. Chem Soc Rev. 2011; 41(6):2283-307. DOI: 10.1039/c1cs15270j. View

18.

Belicky S, Tkac J . Can glycoprofiling be helpful in detecting prostate cancer?. Chem Zvesti. 2016; 69(1):90-111. PMC: 4886849. DOI: 10.1515/chempap-2015-0052. View

19.

Chung C, Kim Y, Shin D, Ryoo S, Hong B, Min D . Biomedical applications of graphene and graphene oxide. Acc Chem Res. 2013; 46(10):2211-24. DOI: 10.1021/ar300159f. View

20.

Alava T, Mann J, Theodore C, Benitez J, Dichtel W, Parpia J . Control of the graphene-protein interface is required to preserve adsorbed protein function. Anal Chem. 2013; 85(5):2754-9. DOI: 10.1021/ac303268z. View