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Determination of Natamycin in Turkish Yoghurt

Overview
Journal Int J Anal Chem
Publisher Wiley
Specialty Chemistry
Date 2016 Jun 9
PMID 27274731
Citations 2
Authors
Dilek Bilgic Alkaya
Ozlem Karalomlu
Affiliations
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Abstract

This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented.

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