Silencing of Carboxypeptidase E Inhibits Cell Proliferation, Tumorigenicity, and Metastasis of Osteosarcoma Cells

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2016 Jun 9

PMID 27274275

Citations 13

Authors

Shuli Fan

Xu Li

Leiming Li

Liguo Wang

Zhangzhen Du

Yan Yang

Jiansong Zhao

Yan Li

Affiliations

Soon will be listed here.

Abstract

Carboxypeptidase E (CPE), a prohormone processing enzyme, has been implicated in the progression of multiple malignancies. However, the biological role and molecular mechanisms of CPE in osteosarcoma remain elusive. In this study, we assessed the effects of CPE on cell proliferation, tumorigenicity, migration, and invasion in osteosarcoma. Our results showed that silencing of CPE significantly inhibited cell proliferation, caused cell cycle arrest at G0/G1 phase, decreased the expression levels of cell cycle protein, cyclin D1, and inhibited tumorigenicity in vivo. Additionally, CPE downregulation repressed the migratory and invasive capacities of osteosarcoma cells in vitro. Furthermore, overexpression of CPE-ΔN (a splice variant of CPE) enhanced the cell growth, migration, and invasion of osteosarcoma cells. It is possible that both CPE forms are involved in the tumorigenesis and development of osteosarcoma, and therefore CPE may provide a promising biological target for osteosarcoma therapy.

Citing Articles

Prognostic and Therapeutic Significance of Cancer-Associated Fibroblasts Genes in Osteosarcoma Based on Bulk and Single-Cell RNA Sequencing Data.

Que Y, Ding T, Wang H, Xu S, He P, Shen Q J Cell Mol Med. 2025; 29(5):e70424.

PMID: 40045162 PMC: 11882394. DOI: 10.1111/jcmm.70424.

CPE correlates with poor prognosis in gastric cancer by promoting tumourigenesis.

Lin J, Huang C, Diao W, Liu H, Lu H, Huang S Heliyon. 2024; 10(9):e29901.

PMID: 38694095 PMC: 11058891. DOI: 10.1016/j.heliyon.2024.e29901.

Identification of the methotrexate resistance-related diagnostic markers in osteosarcoma via adaptive total variation netNMF and multi-omics datasets.

Jiang Z, Han K, Min D, Kong W, Wang S, Gao M Front Genet. 2023; 14:1288073.

PMID: 37937197 PMC: 10625916. DOI: 10.3389/fgene.2023.1288073.

Carboxypeptidase E is a prognostic biomarker co-expressed with osteoblastic genes in osteosarcoma.

Chen D, Wan B, Cheng Y, Luo Y, Bai X, Guo J PeerJ. 2023; 11:e15814.

PMID: 37663298 PMC: 10474831. DOI: 10.7717/peerj.15814.

Carboxypeptidase E and its splice variants: Key regulators of growth and metastasis in multiple cancer types.

Hareendran S, Yang X, Sharma V, Loh Y Cancer Lett. 2022; 548:215882.

PMID: 35988818 PMC: 9532369. DOI: 10.1016/j.canlet.2022.215882.

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