Topical Tetracycline Improves MC903-induced Atopic Dermatitis in Mice Through Inhibition of Inflammatory Cytokines and Thymic Stromal Lymphopoietin Expression

Overview

Journal Chin Med J (Engl)

Specialty General Medicine

Date 2016 Jun 9

PMID 27270547

Citations 9

Authors

Xiao-Jing Liu

Zhang-Lei Mu

Yan Zhao

Jian-Zhong Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Tetracycline (TET) has been found to have both antibiotic and anti-inflammatory properties. The anti-inflammatory effect of topical TET on atopic dermatitis (AD) has not been reported. The purpose of this study was to explore the potential role of topical TET and its anti-inflammatory effects in a mouse model of AD.

Methods: The 2% TET was applied topically to ears of MC903-induced AD-like BALB/c mice once a day. AD-like symptoms and severity were evaluated by assessing skin scoring of dermatitis, ear thickness, and frequency of scratching. Serum IgE and thymic stromal lymphopoietin (TSLP) levels were measured by enzyme-linked immunosorbent assay. Western blot was used for analyzing the expressions of TSLP, protease-activated receptor 2 (PAR2), and nuclear factor-kappa B (NF-κB) in skin lesions. Real-time polymerase chain reaction was performed to assess the mRNA levels of TSLP and inflammatory cytokines including interleukin (IL)-4, IL-13, tumor necrosis factor (TNF)-α, and IL-1β in skin lesions.

Results: Scoring of dermatitis (9.00 ± 0.63 vs. 6.67 ± 1.03, P = 0.001), ear thickness (0.44 ± 0.02 mm vs. 0.40 ± 0.03 mm, P = 0.018), and serum IgE level (421.06 ± 212.13 pg/ml vs. 244.15 ± 121.39 pg/ml, P = 0.047) were all improved in the 2% TET treatment group compared with AD group. Topical TET significantly reduced the serum level of TSLP (119.04 ± 38.92 pg/ml vs. 65.95 ± 54.61 pg/ml, P = 0.011) and both mRNA and protein expressions of TSLP in skin lesions compared with AD group (P = 0.003 and 0.011, respectively), and NF-κB and PAR2 expression in skin lesions were also suppressed (P = 0.016 and 0.040, respectively). Furthermore, expressions of inflammatory cytokines IL-4, IL-13, and TNF-α in skin lesions were down-regulated in 2% TET group compared with AD group (P = 0.035, 0.008, and 0.044, respectively).

Conclusions: Topical TET exerted anti-inflammatory effects through suppression of TSLP and inflammatory cytokines in AD mouse model, suggesting TET as a potential agent for the topical treatment of AD in the future.

Citing Articles

Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice.

Wei M, Yang H, Shao Z, Wan H, Wang Y, Chen W Clin Cosmet Investig Dermatol. 2024; 17:1093-1105.

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NOD/Scid IL2Rγ Mice Reconstituted with PBMCs from Patients with Atopic Dermatitis or Psoriasis Vulgaris Reflect the Respective Phenotype.

Schindler M, Schuster-Winkelmann P, Wess V, Czell S, Rueff F, Wollenberg A JID Innov. 2024; 4(3):100268.

PMID: 38736522 PMC: 11087984. DOI: 10.1016/j.xjidi.2024.100268.

Interplay of cytokines in the pathophysiology of atopic dermatitis: insights from Murin models and human.

Yamamura Y, Nakashima C, Otsuka A Front Med (Lausanne). 2024; 11:1342176.

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IL-32 promotes the occurrence of atopic dermatitis by activating the JAK1/microRNA-155 axis.

Chang J, Zhou B, Wei Z, Luo Y J Transl Med. 2022; 20(1):207.

PMID: 35545774 PMC: 9097387. DOI: 10.1186/s12967-022-03375-x.

Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin.

Wan H, Yang H, Wei M, Chen W Ann Transl Med. 2022; 10(4):209.

PMID: 35280398 PMC: 8908153. DOI: 10.21037/atm-22-282.

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