Developmental Changes in High-affinity Uptake of GABA by Cultured Neurons

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1989 Mar 1

PMID 2725823

Citations 1

Authors

V J Balcar

K L Hauser

H Demieville

Affiliations

Soon will be listed here.

Abstract

High-affinity uptake of [3H]gamma-aminobutyric acid (GABA) was studied in cultures of neonatal rat cortical neurons grown on pre-formed monolayers of non-neuronal (glial) cells. Both the maximum rate (Vmax) and, to a smaller extent, the Km of [3H]GABA uptake increased with time. In addition, in parallel with these changes, 2,4-diaminobutyric acid and cis-3-aminocyclohexane-1-carboxylic acid (ACHC), compounds which are considered typical substrate/inhibitors of GABA uptake in neurons, became progressively stronger inhibitors of [3H]GABA uptake. Consequently, the present results may mean that the studies using uptake of [3H]GABA, [3H]ACHC, or [3H]DABA as a specific marker for GABAergic neurons differentiating during the ontogenetic development of the central nervous system may have to be interpreted with caution.

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PMID: 7957723 DOI: 10.1007/BF00228980.

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